Chemotaxis in microfluidic devices -: a study of flow effects
Chemotaxis in microfluidic devices -: a study of flow effects
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DOI：
10.1039/b801331d
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Bodenschatz, Eberhard
中科院分区：
文献类型：
--
作者：
Beta, Carsten;Froehlich, Toni;Bodenschatz, Eberhard
由于对单个细胞长度尺度上的流动特性和浓度分布的特殊控制，微流体装置的使用在趋化性研究中变得越来越流行。在这些应用中，人们经常忽视的是，附着在微流体室内表面上的细胞是三维物体，会扰乱和扭曲其附近的流场。根据流速和几何形状与流中化学引诱剂扩散时间尺度的相互作用，细胞膜上的浓度分布可能与完美平滑通道中的最佳梯度有很大差异。我们以二维近似分析基础物理，并执行系统数值有限元模拟来表征三维情况并确定最佳流动条件。
The use of microfluidic devices has become increasingly popular in the study of chemotaxis due to the exceptional control of flow properties and concentration profiles on the length scale of individual cells. In these applications, it is often neglected that cells, attached to the inner surfaces of the microfluidic chamber, are three-dimensional objects that perturb and distort the flow field in their vicinity. Depending on the interplay of flow speed and geometry with the diffusive time scale of the chemoattractant in the flow, the concentration distribution across the cell membrane may differ strongly from the optimal gradient in a perfectly smooth channel. We analyze the underlying physics in a two-dimensional approximation and perform systematic numerical finite element simulations to characterize the three-dimensional case and to identify optimal flow conditions.