An arbuscular mycorrhizal fungus ameliorates plant growth and hormones after moderate root damage due to simulated coal mining subsidence: a microcosm study
An arbuscular mycorrhizal fungus ameliorates plant growth and hormones after moderate root damage due to simulated coal mining subsidence: a microcosm study
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DOI：
10.1007/s11356-019-04559-7
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发表时间：
2019-04-01
影响因子：
5.8
通讯作者：
Sun, Jinhua
中科院分区：
文献类型：
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作者：
Bi, Yinli;Xiao, Li;Sun, Jinhua
丛枝菌根真菌（AMF）是专性植物根系共生体，为寄主植物提供一系列益处，如改善营养获取和抵抗病原体和非生物胁迫。然而，它们是否能增强因过度采煤引起的沉陷而受损的植物根系的功能，尚未得到很好的探讨。本研究通过模拟采掘沉陷，以苔藓漏斗菌（FM）为试验真菌，研究了不同程度根系损伤胁迫下AMF对玉米（Zea mays L.）生长和激素的影响。结果表明：在相同的模拟采动沉陷条件下，FM处理的植株比未FM处理的植株有更多的芽、根、菌根定植和更高的菌丝密度；此外，FM处理植株的茎部和根系中N、P、K、Ca、Mg含量较高，根系中吲哚-3-乙酸、赤霉素（GA）和细胞分裂素（CTK）含量较高，表明菌根关联促进了植株生物量和养分吸收。当开采沉陷造成的根系损伤超过一半时，调频处理不再有效。土壤SOC、AK和TG是影响GA、CTK、IAA和ABA的关键因子，AMF可以通过菌丝直接改变植物激素，也可以通过改变根系损伤胁迫下土壤理化性质间接改变植物激素。总的来说，我们的研究结果为评估AMF对采煤沉陷的生物复垦效果提供了基线数据。
Arbuscular mycorrhizal fungi (AMF) are obligate plant root symbionts delivering a range of benefits to the host plant such as improved nutrient acquisition and resistance to pathogens and abiotic stress. However, whether they can enhance the function of plant root systems damaged due to subsidence caused by excessive coal mining has not been well explored. In the present study, we investigated the effects of AMF using Funneliformis mosseae (FM) as the test fungus on maize (Zea mays L.) growth and hormone levels under different levels of root damage stress by simulating mining subsidence. The results show that plants treated with FM had more shoots, roots, mycorrhizal colonization and higher hyphal density than those without FM under the same simulated mining-induced subsidence conditions. In addition, plants treated with FM also possessed higher N, P, K, Ca, and Mg contents in the shoots and the roots and higher indole-3-acetic acid, gibberellin (GA), and cytokinin (CTK) contents in the roots, indicating that the mycorrhizal association promoted plant biomass and nutrient uptake. FM treatment was no longer beneficial when root damage due to mining-induced subsidence affected more than half of the roots. Soil SOC, AK, and TG were identified as key factors affecting GA, CTK, IAA, and ABA, and AMF can alter plant hormones directly via the hyphae and indirectly by altering soil physicochemical properties under root damage stress. Overall, our results provide baseline data for assessing the biological reclamation effects of AMF on coal mining-induced subsidence.