STUDIES ON LYMPHOCYTES OF SHEEP .3. DESTINATION OF LYMPH-BORNE IMMUNOBLASTS IN RELATION TO THEIR TISSUE OF ORIGIN
STUDIES ON LYMPHOCYTES OF SHEEP .3. DESTINATION OF LYMPH-BORNE IMMUNOBLASTS IN RELATION TO THEIR TISSUE OF ORIGIN
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DOI：
10.1002/eji.1830070108
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发表时间：
1977-01-01
影响因子：
5.4
通讯作者：
ORLANS, E
中科院分区：
文献类型：
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作者：
HALL, JG;HOPKINS, J;ORLANS, E
淋巴来源的免疫母细胞体外用~(125)I-脱氧尿苷标记，洗涤后静脉注射返回。注射到采集它们的绵羊身上。20小时后处死绵羊，通过测定各脏器的放射性来确定免疫母细胞的分布。来自外周体淋巴的免疫母细胞主要流向脾、肺等器官，而来自肠淋巴的免疫母细胞主要流向小肠。肠道免疫母细胞对肠道的这种归宿能力也在胎儿宫内无菌环境中得到了证明；显然，免疫母细胞的迁移行为，就像小淋巴细胞一样，主要不是由抗原驱动的。设计了一种收集外周(即肠系膜结节的传入)肠淋巴的技术，其中含有来自其他组织的外周淋巴中出现的小淋巴细胞的10-20倍。来自外周肠道淋巴的免疫母细胞也归巢于肠道。通过制备淋巴细胞洗涤剂提取液、免疫过氧化物酶技术在细胞膜上的应用以及免疫母细胞体外掺入~(14)C标记氨基酸的方法，研究了免疫母细胞免疫球蛋白[Ig]的含量。来自体液和肠淋巴的免疫母细胞含有并产生Ig G和Ig M，但许多肠道免疫母细胞含有并产生Ig A。目前尚不清楚这种免疫球蛋白是否介导了免疫母细胞向肠道的外渗。淋巴样细胞可能有两条主要的迁移途径：一条是通过肠道相关的淋巴组织，另一条是通过体脾淋巴组织。
Lymph-borne immunoblasts were labeled in vitro with [125I]iodo-deoxy-uridine, washed and returned by i.v. injection to the sheep from which they were collected. Twenty hours later the sheep were killed and the distribution of the immunoblasts was determined by assaying the radioactivity in various organs. Immunoblasts from the efferent lymph of peripheral somatic lymph nodes (PSLN) went mainly to the spleen, lungs and other PSLN, while immunoblasts from intestinal lymph went mainly to the small gut. This ability of intestinal immunoblasts to home to the gut was demonstrated also in the sterile environment of fetuses in utero; apparently the migratory behavior of immunoblasts, like that of small lymphocytes, is not primarily antigen-driven. A technique was devised for the collection of peripheral (i.e., afferent to the mesenteric node) intestinal lymph which contained 10-20 times the numbers of small lymphocytes that occur in the peripheral lymph from other tissues. Immunoblasts from peripheral intestinal lymph also homed to the gut. The immunoglobulin [Ig] content of immunoblasts was studied by making detergent extracts of lymph cells, by applying immuno-peroxidase techniques to cell films and by investigating the incorporation of 14C-labeled amino acids into Ig by immunoblasts in vitro. Immunoblasts from somatic and intestinal lymph contained and made IgG and IgM, but many intestinal immunoblasts contained and made IgA. It is not known whether this Ig mediates the extravasation of immunoblasts into the gut. There may be 2 major migratory pathways for lymphoid cells; one through the gut-associated lymphoid tissue and the other through the somatic-splenic lymphoid tissues.