Intracellular mediators of goblet cell degranulation in isolated pancreatic ducts.
Intracellular mediators of goblet cell degranulation in isolated pancreatic ducts.
复制标题
离体胰管中杯状细胞脱粒的细胞内介质。
DOI：
10.1006/bbrc.1994.2682
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发表时间：
1994
影响因子：
3.1
通讯作者：
Hootman,SR
中科院分区：
文献类型：
--
作者：
Wilkins,TJ;deOndarza,J;Hootman,SR
研究了特定细胞内信号通路在激动剂诱导的豚鼠胰管杯状细胞脱颗粒中的作用。卡巴胆碱、血管活性肠肽(VIP)、钙离子载体A23187、佛波酯12-O-十四酰佛波醇13-乙酸酯(TPA)、环磷酸腺苷类似物Sp-5，6-DCL-cBIMPS和Forsklin均可引起离体管中杯状细胞脱颗粒。冈田酸、细胞松驰素D和诺康唑不能抑制氨基甲胆碱引起的脱颗粒。这些结果表明，至少有两条主要的信号通路参与了胰管杯状细胞的分泌。
The involvement of particular intracellular signalling pathways in agonist-evoked degranulation of guinea pig pancreatic duct goblet cells was investigated. Carbachol, vasoactive intestinal peptide (VIP), calcium ionophore A23187, phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), cyclic AMP analogue Sp-5,6-DCl-cBIMPS and forskolin each caused degranulation of goblet cells in isolated ducts. Degranulation induced by carbachol was not inhibited by okadaic acid, cytochalasin-D or nocodazole. These results indicate that at least two major signalling pathways are involved in pancreatic duct goblet cell secretion.