2-PHOTON SCANNING PHOTOCHEMICAL MICROSCOPY - MAPPING LIGAND-GATED ION-CHANNEL DISTRIBUTIONS
2-PHOTON SCANNING PHOTOCHEMICAL MICROSCOPY - MAPPING LIGAND-GATED ION-CHANNEL DISTRIBUTIONS
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DOI：
10.1073/pnas.91.14.6629
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发表时间：
1994-07-05
影响因子：
11.1
通讯作者：
DENK, W
中科院分区：
文献类型：
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作者：
DENK, W
负责接受整个神经系统突触传递的离子亲膜受体的位置和密度，对于理解神经回路的功能是至关重要的。结果表明，通过双光子吸收介导的光激活，“笼中”神经递质的高度局域化释放可以结合记录诱导的全细胞电流来确定配基门控离子通道的分布。这项技术潜在地足够敏感，可以用衍射受限的空间分辨率来检测单个通道。用烟碱激动剂氨基甲酰胆碱的可光激活前体获得培养的BC3H1细胞上烟碱型乙酰胆碱受体分布的图像。
The locations and densities of ionotropic membrane receptors, which are responsible for receiving synaptic transmission throughout the nervous system, are of prime importance in understanding the function of neural circuits. It is shown that the highly localized liberation of ''caged'' neurotransmitters by two-photon absorption-mediated photoactivation can be used in conjunction with recording the induced whole-cell current to determine the distribution of ligand-gated ion channels. The technique is potentially sensitive enough to detect individual channels with diffraction-limited spatial resolution. Images of the distribution of nicotinic acetylcholine receptors on cultured BC3H1 cells were obtained using a photoactivatable precursor of the nicotinic agonist carbamoylcholine.