Determination of deoxynivalenol and nivalenol chemotypes of Fusarium graminearum isolates from China by PCR assay
Determination of deoxynivalenol and nivalenol chemotypes of Fusarium graminearum isolates from China by PCR assay
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DOI：
10.1111/j.1439-0434.2007.01270.x
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发表时间：
2007-08-01
影响因子：
1.5
通讯作者：
Wang, S.
中科院分区：
文献类型：
--
作者：
Ji, L.;Cao, K.;Wang, S.
禾谷镰刀菌（Fusarium graminearum）是我国禾谷类赤霉病和玉米穗轴腐病的主要病原菌。255华氏度从河北、黑龙江和湖北等气候条件和种植制度不同的省份的小麦、大麦和玉米中分离到禾谷镰刀菌（graminearum）菌株。经鉴定为F.禾谷镰刀菌（F. graminearum）的PCR检测。禾谷镰刀菌种特异性引物Fg 16 F/Fg 16 R。在中国已鉴定出两个群体7 C1和6A 5。湖北省沿着地区以6A 5种群为主，而北方黑龙江省和河北省则以7 C1种群为主。根据真菌毒素生物合成途径相关基因Tri 13和Tri 3的序列，采用PCR方法检测3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）、15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON）和雪腐镰刀菌烯醇（NIV）的化学型。F.禾谷镰刀菌，15-ADON化学型占主导地位的整体。湖北省3-ADON型比例较高，黑龙江省和河北省15-ADON型比例较高。
Fusarium graminearum is the main causative agent of cereal scab and maize cob rot in China. Two hundred and fifty-five F. graminearum isolates were obtained from wheat, barley and maize from Hebei, Heilongjiang and Hubei, provinces with distinct climate conditions and cropping systems. The isolates were confirmed to be F. graminearum by polymerase chain reaction (PCR) assay using F. graminearum species-specific primers Fg16F/Fg16R. Two populations, 7C1 and 6A5, were identified to exist in China. The 6A5 population was predominant in Hubei in central China along the Yangtze River, whereas the 7C1 population was predominant in Heilongjiang and Hebei in northern China. Based on sequences of Tri13 and Tri3, genes involved in the mycotoxin biosynthetic pathway, PCR assays were used to detect 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (l5-ADON) and nivalenol (NIV) chemotypes. All three chemotypes of F. graminearum were identified, with 15-ADON chemotype predominating overall. A greater proportion of 3-ADON chemotype was found in Hubei, whereas a greater proportion of 15-ADON was found in Heilongjiang and Hebei.