Mated Drosophila melanogaster females require a seminal fluid protein, Acp36DE, to store sperm efficiently.
Mated Drosophila melanogaster females require a seminal fluid protein, Acp36DE, to store sperm efficiently.
复制标题
交配的雌性黑腹果蝇需要精液蛋白 Acp36DE 来有效储存精子。
DOI：
10.1093/genetics/153.2.845
复制
发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Wolfner,MF
中科院分区：
文献类型：
--
作者：
Neubaum,DM;Wolfner,MF
许多动物的交配雌性都储存精子。精子储存深刻地影响着女性后代的数量、时间和父权。为了研究黑腹果蝇精子储存的机制，我们生成并分析了Acp36DE的突变。Acp36DE是一种雄性精液蛋白，其在交配雌性中的定位提示其在精子储存中起作用。我们报道雄性来源的Acp36DE对于雌性有效的精子储存至关重要。Acp36DE1（无效）突变雄性产生并转移正常数量的精子和精液蛋白。然而，与对照雌性相比，acp36de1雄性的配偶只储存了15%的精子，产生了10%的成年后代。此外，没有Acp36DE，交配后的雌性无法维持较高的产卵率和降低的接受性，这些行为的持续（而不是开始）通常取决于储存精子的存在。先前的研究表明，输卵管中的屏障将精子和Acp36DE限制在存储器官附近的有限区域。我们发现Acp36DE不是屏障形成所必需的，但Acp36DE和屏障都是最大精子储存所必需的。Acp36DE与精子密切相关。我们的研究结果表明，Acp36DE对精子的初始储存至关重要，它也可能影响储存精子的排列和保留。
Mated females of many animal species store sperm. Sperm storage profoundly influences the number, timing, and paternity of the female’s progeny. To investigate mechanisms for sperm storage in Drosophila melanogaster, we generated and analyzed mutations in Acp36DE. Acp36DE is a male seminal fluid protein whose localization in mated females suggested a role in sperm storage. We report that male-derived Acp36DE is essential for efficient sperm storage by females. Acp36DE1(null) mutant males produced and transferred normal amounts of sperm and seminal fluid proteins. However, mates of Acp36DE1males stored only 15% as many sperm and produced 10% as many adult progeny as control-mated females. Moreover, without Acp36DE, mated females failed to maintain an elevated egg-laying rate and decreased receptivity, behaviors whose persistence (but not initiation) normally depends on the presence of stored sperm. Previous studies suggested that a barrier in the oviduct confines sperm and Acp36DE to a limited area near the storage organs. We show that Acp36DE is not required for barrier formation, but both Acp36DE and the barrier are required for maximal sperm storage. Acp36DE associates tightly with sperm. Our results indicate that Acp36DE is essential for the initial storage of sperm, and that it may also influence the arrangement and retention of stored sperm.