PREPARATION, CRYSTALLINE-STRUCTURE, AND SPECTRAL PROPERTIES OF FLUORESCENT-PROBE 4,4=-BIS-1-PHENYLAMINO-8-NAPHTHALENESULFONATE
PREPARATION, CRYSTALLINE-STRUCTURE, AND SPECTRAL PROPERTIES OF FLUORESCENT-PROBE 4,4=-BIS-1-PHENYLAMINO-8-NAPHTHALENESULFONATE
复制标题
DOI：
10.1021/ja00482a026
复制
发表时间：
1978-01-01
影响因子：
15
通讯作者：
PAUL, IC
中科院分区：
文献类型：
--
作者：
FARRIS, FJ;WEBER, G;PAUL, IC
本文介绍了1-苯氨基和1-甲苯氨基苯磺酸共价二聚体的制备和纯化方法。X-射线分析表明，前者的K盐晶体(双-ANS)为4，4‘’-bis-1-phenylaminonaphthalene-8-sulfonate.根据核磁共振谱和光学性质的相似性，确定第二个二聚体为4，4‘’-bis-1-toluidylaminonaphthalene-8-sulfonic酸。给出了核磁共振谱、摩尔吸收系数、荧光寿命和由X射线数据得出的详细结构参数。简要讨论了这些化合物在蛋白质溶液和晶体中作为荧光探针的一些用途。
A method for the preparation and purification of the covalent dimers of 1-phenylamino- and 1-toluidylaminonaphthalenesulfonic acids is described. X-ray analysis of the crystals of the K salt of the former (bis-ANS) showed this to be 4,4''-bis-1-phenylaminonaphthalene-8-sulfonate. By similarity of the NMR spectra and optical properties the second dimer is identified as 4,4''-bis-1-toluidylaminonaphthalene-8-sulfonic acid. NMR spectra, molar absorption coefficients, fluorescent lifetimes, and the detailed structural parameters derived from the X-ray data are presented. Some of the uses of the compounds as fluorescent probes in solutions and crystals of proteins are briefly discussed.