Gene expression differentiation in the reproductive tissues of Drosophila willistoni subspecies and their hybrids
Gene expression differentiation in the reproductive tissues of Drosophila willistoni subspecies and their hybrids
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DOI：
10.1111/mec.16941
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发表时间：
2023-04-16
影响因子：
4.9
通讯作者：
Civetta，Alberto
中科院分区：
文献类型：
--
作者：
Ranz，Jose M.;Go，Alwyn C.;Civetta，Alberto
早期谱系多样化对于理解哪些突变事件导致物种分化至关重要。特别是，种间杂种中的基因失调可以了解哪些基因和途径导致杂种功能障碍。在果蝇杂交种中，调控进化如何影响不同的生殖组织仍有待研究。在这里，我们在果蝇中生成了新的基因组组装和注释，并分析了两种异域进化的果蝇之间的转录组差异模式。 willistoni亚种，其雄性不育和雌性可育杂交后代跨越睾丸、雄性副腺和卵巢。转录组分歧的模式和调控进化的模式是组织特异性的。尽管没有迹象表明杂种睾丸中存在细胞类型差异，但该组织在亚种之间以及亲本和杂种之间表现出最大程度的表达差异。没有检测到杂种雄性组织中异常剂量补偿的证据，也没有发现 theD 的新臂和祖先臂的差异作用。威利斯托尼X染色体。与常染色体相比，X染色体在睾丸中似乎富集了越界表达的基因，尽管它在亚种之间的表达差异最小。越界表达基因的精细基因组聚类的证据表明染色质结构在杂种基因失调中的作用。最后，不育雄性后代睾丸中越界表达的基因富含 GO 术语，这些术语通常与精子功能无关，而是暗示生殖组织的异常发育。我们对最近分歧的 D 之间的转录组差异进行了全面的组织水平描述。 willistoni亚种及其杂种为物种形成过程中的早期调控变化提供了更细致的视角。
Early lineage diversification is central to understand what mutational events drive species divergence. Particularly, gene misregulation in interspecific hybrids can inform about what genes and pathways underlie hybrid dysfunction. InDrosophilahybrids, how regulatory evolution impacts different reproductive tissues remains understudied. Here, we generate a new genome assembly and annotation inDrosophila willistoniand analyse the patterns of transcriptome divergence between two allopatrically evolvedD. willistonisubspecies, their male sterile and female fertile hybrid progeny across testis, male accessory gland, and ovary. Patterns of transcriptome divergence and modes of regulatory evolution were tissue‐specific. Despite no indication for cell‐type differences in hybrid testis, this tissue exhibited the largest magnitude of expression differentiation between subspecies and between parentals and hybrids. No evidence for anomalous dosage compensation in hybrid male tissues was detected nor was a differential role for the neo‐ and the ancestral arms of theD. willistoni Xchromosome. Compared to the autosomes, theXchromosome appeared enriched for transgressively expressed genes in testis despite being the least differentiated in expression between subspecies. Evidence for fine genome clustering of transgressively expressed genes suggests a role of chromatin structure on hybrid gene misregulation. Lastly, transgressively expressed genes in the testis of the sterile male progeny were enriched for GO terms not typically associated with sperm function, instead hinting at anomalous development of the reproductive tissue. Our thorough tissue‐level portrait of transcriptome differentiation between recently divergedD. willistonisubspecies and their hybrids provides a more nuanced view of early regulatory changes during speciation.