Electronic PCR: bridging the gap between genome mapping and genome sequencing
Electronic PCR: bridging the gap between genome mapping and genome sequencing
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DOI：
10.1016/s0167-7799(98)01232-3
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发表时间：
1998-11-01
影响因子：
17.3
通讯作者：
Schuler, GD
中科院分区：
文献类型：
--
作者：
Schuler, GD
人类基因组计划历史上的一个关键事件是决定使用序列标记位点（STS）作为基因组作图的共同标志。经过几年构建越来越详细的基于 STS 的图谱，最近重点已转向大规模基因组测序。当数据从测序管道中出现时，一种称为“电子 PCR”的计算程序允许 STS 标志被揭示，从而弥合作图和测序活动之间的差距。
A crucial event in the history of the Human Genome Project was the decision to use sequence-tagged sites (STSs) as common landmarks for genomic mapping. Following several years of constructing STS-based maps of ever-increasing detail, the emphasis has recently shifted towards large-scale genomic sequencing. A computational procedure called 'electronic PCR' allows STS landmarks to be revealed as data emerge from the sequencing pipeline, thereby bridging the gap between mapping and sequencing activities.