Complete mitochondrial genome of the Yunnanilus Sichuanensis and phylogenetic analysis
Complete mitochondrial genome of the Yunnanilus Sichuanensis and phylogenetic analysis
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DOI：
10.1080/23802359.2019.1579073
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发表时间：
2019-01-02
影响因子：
0.5
通讯作者：
Wen, Zhengyong
中科院分区：
文献类型：
--
作者：
Zou, Yuanchao;Zhang, Junying;Wen, Zhengyong
本研究利用新一代测序技术首次测定了四川云南鳅（Cypriniformes：Nemachelinae）线粒体基因组全序列。有丝分裂基因组长度为16，571 bp，包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因、1个置换环（D-loop）位点和轻链（OL）区的复制起点。除ND 6和8个tRNA基因在轻链上编码外，大部分基因在重链上编码。有丝分裂基因组的组织和基因顺序与典型的脊椎动物有丝分裂基因组相似。总碱组成为26.96%A、28.62%T、25.75%C和18.67%G，其中AT为55.58%AT。基于13个蛋白质编码基因的核苷酸序列，采用邻区连接和最大似然分析两种不同的方法进行系统发育分析。sichuanensis与Y. jinxiensis聚为一个分支，同时与美丽高原鳅形成姐妹群。本研究丰富了云南线鳅属鱼类的有丝分裂基因组数据，为线鳅亚科鱼类的形态分类和生物起源研究提供了重要信息。
The complete mitochondrial genome of the Yunnanilus sichuanensis (Cypriniformes: Nemacheilinae) was first sequenced using the next-generation sequencing in this study. The mitogenome was 16,571 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 displacement loop (D-loop) locus, and an origin of replication on the light-strand (OL) region. Most of the genes were encoded on the heavy strand except ND6 and 8 tRNA genes encoded on the light strand. The mitogenome organization and gene order were similar to a typical vertebrate mitogeneome. The overall base composition was 26.96% A, 28.62% T, 25.75% C and 18.67% G, with 55.58% AT. Phylogenetic analysis based on the 13 protein-coding genes nucleotide sequences with two different methods (neighbour-joining and maximum likelihood analysis) showed that Y. sichuanensis was nearest to Y. jinxiensis, coming together into a branch, meanwhile together with Triplophysa venusta formed a sister group. This study enriched the data of the mitogenome of the genus Yunnanilus, and provide an important information for morphological classification of Nemacheilinae species and the study of biological origin.