Distribution and colocalization of NGF and GDNF family ligand receptor mRNAs in dorsal root and nodose ganglion neurons of adult rats
Distribution and colocalization of NGF and GDNF family ligand receptor mRNAs in dorsal root and nodose ganglion neurons of adult rats
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DOI：
10.1016/s0169-328x(02)00584-3
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发表时间：
2003-01-31
期刊：
影响因子：
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通讯作者：
Senba, E
中科院分区：
文献类型：
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作者：
Kashiba, H;Uchida, Y;Senba, E
为了了解初级感觉神经元对神经营养因子的依赖性，我们研究了神经生长因子（NGF）和胶质细胞源性神经营养因子（GDNF）家族配体受体的mRNA在成年大鼠背根神经节（DRG）和结状神经节（NG）神经元中的分布和共定位。大约35%、10%和20%的腰DRG神经元分别表达trkA、trkB和trkC mRNA。在大约60%的DRG神经元中观察到c-ret（GDNF家族配体的常见信号受体）的信使RNA信号，其中一些神经元表达trkA、trkB或trkC mRNA。观察到的大多数（97%）DRG神经元对这四种mRNA中的至少一种呈阳性。大约50%、20%和20%的DRG神经元分别表达GDNF家族受体α 1（GFR α 1）、GFR α 2和GFR α 3 mRNA，并且这些神经元中的大多数对c-ret mRNA呈阳性。有趣的是，GFR α 2和GFR α 3 mRNA信号经常出现在相同的神经元中，而这些神经元缺乏GFR α 1 mRNA信号。另一方面，98%的NG神经元表达trkB mRNA，30-40%的NG神经元共表达c-ret和GFR α 1 mRNA。然而，仅在少数NG神经元中观察到其他受体（TrkA、TrkC、GFR α 2、GFR α 3）的mRNA信号。这些结果表明，所有的DRG神经元在成年大鼠依赖于至少一个的NGF和GDNF家族的配体，和一些DRG神经元依赖于两个或更多的配体。相反，NG神经元被认为分为两大类;一类依赖于脑源性神经营养因子（BDNF）/神经营养因子-4/5（NT-4/5），另一类依赖于BDNF/NT-4/5和GDNF。(C)2002 Elsevier Science B. V.保留所有权利。
To understand the dependence of primary sensory neurons on neurotrophic factors, we examined the distribution and colocalization of mRNAs for receptors of nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) family ligands in dorsal root ganglion (DRG) and nodose ganglion (NG) neurons of adult rats by in situ hybridization (ISH) histochemistry using serial sections. About 35, 10, and 20% of the lumbar DRG neurons expressed trkA, trkB and trkC mRNAs, respectively. Messenger RNA signals for c-ret, a common signaling receptor of GDNF family ligands, were seen in about 60% of DRG neurons, and some of these neurons expressed trkA, trkB, or trkC mRNAs. Most (97%) of the DRG neurons observed were positive to at least one of these four mRNAs. About 50, 20, and 20% of DRG neurons expressed GDNF family receptor alpha1 (GFR alpha1), GFR alpha2, and GFR alpha3 mRNAs, respectively, and most of these neurons were positive to c-ret mRNA. Interestingly, GFR alpha2 and GFR alpha3 mRNA signals were frequently seen in the same neurons, which lack GFR alpha1 mRNA signals. On the other hand, 98% of NG neurons expressed trkB mRNA and 30-40% of NG neurons co-expressed c-ret and GFR alpha1 mRNAs. However, mRNA signals for other receptors (TrkA, TrkC, GFR alpha2, GFR alpha3) were seen in only a few NG neurons. These findings suggest that all the DRG neurons in adult rats depend on at least one of the NGF and GDNF family ligands, and that some DRG neurons depend on two ligands or more. In contrast, NG neurons were suggested to be divided into two major groups; one group depends on brain-derived neurotrophic factor (BDNF)/neurotrophin-4/5 (NT-4/5), and the other depends on both BDNF/NT-4/5 and GDNF. (C) 2002 Elsevier Science B.V. All rights reserved.