In Vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells
In Vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells
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DOI：
10.1016/j.bbrc.2005.12.057
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发表时间：
2006-02-10
影响因子：
3.1
通讯作者：
Torii, R
中科院分区：
文献类型：
--
作者：
Abdelalim, EM;Takada, T;Torii, R
ES细胞来源的心肌细胞的功能特征对其分化调控和应用于细胞治疗具有重要意义。心肌细胞的重要功能之一是产生心钠素和脑利钠肽(分别为ANP和BNP)，它们具有重要的内分泌、自分泌和旁分泌功能。在本研究中，我们重点研究了从体外培养的猴ES细胞分化为心肌细胞的功能，并研究了ANP和BNP的表达。RT-PCR和免疫细胞化学显示自发收缩细胞有结节样动作电位，ANP和BNP表达。有趣的是，ANP和BNP在核周区域以免疫反应颗粒的形式表达，这些信号似乎与反高尔基网络共定位。这些结果表明，猴ES细胞在体外可以分化为具有功能特性的心肌细胞，可以作为研究早期心肌发生机制和功能的有用模型。(C)2005 Elsevier Inc.保留所有权利。
Functional characterization of ES cell-derived cardiomyocytes is important for differentiation control and application to the cell therapy. One of the crucial functions of cardiomyocytes is a production of atrial and brain natriuretic peptides (ANP and BNP, respectively), which have important endocrine, autocrine, and paracrine functions. In this study, we focused on the functional aspect of the cardiomyocytes differentiated from monkey ES cells in vitro and investigated the expression of ANP and BNP. Spontaneously contracting cells showed nodal-like action potentials, and expression of ANP and BNP by RT-PCR and immunocytochemistry. Interestingly, ANP and BNP expressions were detected as immunoreactive granules in the perinuclear area and these signals appeared to co-localize with trans-Golgi network. These findings suggest that monkey ES cells were able to differentiate into cardiomyocytes with functional characteristics in vitro and therefore can be used as a useful model to study mechanisms and functions in early cardiogenesis. (c) 2005 Elsevier Inc. All rights reserved.