Increased expression of the rat myometrial oxytocin receptor messenger ribonucleic acid during labor requires both mechanical and hormonal signals
Increased expression of the rat myometrial oxytocin receptor messenger ribonucleic acid during labor requires both mechanical and hormonal signals
复制标题
DOI：
10.1095/biolreprod59.5.1055
复制
发表时间：
1998-11-01
影响因子：
3.6
通讯作者：
Lye, SJ
中科院分区：
文献类型：
--
作者：
Ou, CW;Chen, ZQ;Lye, SJ
我们研究了大鼠子宫肌层催产素受体（OTR）mRNA的表达及其孕激素和机械牵张的调节。使用半定量逆转录-聚合酶链反应的方法，OTR mRNA被发现突然增加，在自然分娩的发病条件。从妊娠第20天开始给予孕酮（4 mg/天）可阻断这种增加。第17天卵巢切除术后96 h诱导早产，术后48和96 h子宫肌层OTR mRNA水平显著增加。孕激素阻断了早产和子宫肌层OTR表达的增加。为了研究牵张对子宫肌层OTR mRNA表达的影响，单侧妊娠大鼠进行假手术或在非妊娠子宫角放置一根管以扩张子宫肌层。在第20天，拉伸对非妊娠角中的OTR表达没有影响。在分娩过程中，OTR mRNA在妊娠角和非妊娠牵拉角中高度表达。与此相反，水平仍然很低，在nongravid未拉伸的角。这些结果表明，大鼠子宫肌层OTR mRNA的表达在妊娠和分娩过程中的机械和内分泌信号之间的协调相互作用的调节。
We investigated the expression of the mRNA encoding the oxytocin receptor (OTR) in rat myometrium throughout gestation and its regulation by progesterone and mechanical stretch. Using a semiquantitative reverse transcription-polymerase chain reaction approach, OTR mRNA was found to increase abruptly at the onset of spontaneous labor at term. Progesterone (4 mg/ day) starting on Day 20 of gestation blocked this increase. Ovariectomy on Day 17 induced preterm labor 96 h after surgery and a significant increase in myometrial OTR mRNA levels 48 and 96 h after surgery. Both preterm labor and the rise in myometrial OTR expression were blocked by progesterone. To investigate the effects of stretch on myometrial OTR mRNA expression, unilaterally pregnant rats underwent either sham operation or placement of a tube in the nongravid uterine horn to distend the myometrium. On Day 20, stretch had no effect on OTR expression in the nongravid horns. During labor, OTR mRNA was highly expressed in the gravid horns as well as the nongravid stretched horns. In contrast, the level remained low in the nongravid unstretched horns. These results indicate that expression of rat myometrial OTR mRNA during pregnancy and labor is regulated by coordinated interactions between mechanical and endocrine signals.