Mechanistic Insight into a Novel Ultrasensitive Nicotine Assay Base on High-Efficiency Quenching of Gold Nanocluster Cathodic Electrochemiluminescence
Mechanistic Insight into a Novel Ultrasensitive Nicotine Assay Base on High-Efficiency Quenching of Gold Nanocluster Cathodic Electrochemiluminescence
复制标题
基于金纳米团簇阴极电化学发光高效猝灭的新型超灵敏尼古丁测定的机理洞察
DOI：
10.1021/acs.analchem.0c02500
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发表时间：
2020-08-18
影响因子：
7.4
通讯作者：
Chen，Wei
中科院分区：
文献类型：
--
作者：
Huang，Zhongnan;Li，Zhenglian;Chen，Wei
由于尼古丁对人类健康的有害影响，监测人体体液中的尼古丁浓度至关重要。研究表明，尼古丁可以高效地猝灭金纳米团簇的阴极电致化学发光。利用各种实验手段和理论计算对尼古丁的电化学发光猝灭机理进行了详细的研究。结果表明，K2S2O8产生的强氧化中间体SO4·-可以氧化尼古丁，导致ECL发射猝灭。在AuNCs/K2S2O8体系高效电致发光猝灭的基础上，提出了一种可回收、超灵敏、高选择性的尼古丁电致发光检测平台。即使在没有任何复杂的信号放大技术的情况下，用于尼古丁检测的电致发光传感器也显示出前所未有的低检测下限7×10-13M(S/N=3)和8个数量级的宽的线性范围。最值得注意的是，它可以成功地用于检测人体尿样中的尼古丁。这有望促进尼古丁相关疾病的研究和应用。我们相信，建议的ECL平台在生物医药领域和烟草行业具有巨大的商业化前景。
Monitoring nicotine concentrations in human fluids is extremely crucial owing to the harmful effect of nicotine on human health. Herein, it is shown that nicotine could quench the cathodic electrochemiluminescence (ECL) of gold nanoclusters (AuNCs) with high efficiency. The ECL quenching mechanism of nicotine was studied in detail using various experimental tools and theoretical calculations. It was concluded that the strongly oxidizing intermediate SO4•–, produced from K2S2O8, could oxidized nicotine, resulting in ECL emission quenching. On the basis of this high-efficiency ECL quenching of the AuNCs/K2S2O8system, a recyclable, ultrasensitive, and selective ECL sensing platform for nicotine detection was proposed. Even in the absence of any complex signal amplification techniques, the ECL sensor for nicotine detection showed an unprecedentedly low detection limit of 7.0 × 10–13M (S/N = 3) and a wide linear range over 8 orders of magnitude. Most remarkably, it could be successfully used for nicotine detection in human urine samples. This is expected to promote the investigations and applications on nicotine-related diseases. We believe that the proposed ECL platform can hold great prospects for commercialization in biomedical fields and tobacco industries.