PcrA/UvrD/Rep DNA helicases in bacterial genomes
PcrA/UvrD/Rep DNA helicases in bacterial genomes
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DOI：
10.1016/j.bse.2007.08.001
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发表时间：
2008-02-01
影响因子：
1.6
通讯作者：
Chene, Patrick
中科院分区：
文献类型：
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作者：
Chene, Patrick
解旋酶利用核苷酸水解释放的能量解开核酸，参与核酸新陈代谢的许多方面。PcrA/UvrD/Rep亚家族的各种DNA解旋酶对于不同的病原菌的生存是必不可少的，我们最近发现它们可以被合成的小分子抑制。总而言之，这表明这些酶是潜在的新药物靶点。由于对这些酶在细菌基因组中的存在知之甚少，本研究对99个细菌基因组进行了分析。这一分析揭示了在细菌中发现了哪些和多少这样的酶，但更重要的是，它确定了其中几种酶是潜在的药物靶标。此外，这项工作还鉴定了几种蛋白质，这里称为Purl，它们与PcrA/UvrD/Rep蛋白有很高的同源性，可能形成这个解旋酶亚家族中的一个额外的基团。(C)2007爱思唯尔有限公司。保留所有权利。
Helicases, which utilize the energy liberated by the hydrolysis of nucleotides to unwind nucleic acids, are involved in many aspects of nucleic acid metabolism. Various DNA helicases from the PcrA/UvrD/Rep subfamily are essential for the survival of different pathogenic bacteria and we have recently shown that they can be inhibited with small synthetic molecules. Altogether this suggests that these enzymes are potential new drug targets. Since little is known about the presence of these enzymes in bacterial genomes, 99 bacterial genomes were analyzed in the present study. This analysis reveals which and how many of these enzymes are found in bacteria, but more important, it identifies several of these enzymes as potential drug target candidates. In addition, this work identifies several proteins, called here PURL, that have a high homology with the PcrA/UvrD/Rep proteins and that may form an additional group in this helicase subfamily. (C) 2007 Elsevier Ltd. All rights reserved.