Inhibition of HIV-1 infection by aqueous extracts of Prunella vulgaris L.
Inhibition of HIV-1 infection by aqueous extracts of Prunella vulgaris L.
复制标题
DOI：
10.1186/1743-422x-8-188
复制
发表时间：
2011-04-23
期刊：
影响因子：
4.8
通讯作者：
Maury W
中科院分区：
文献类型：
--
作者：
Oh C;Price J;Brindley MA;Widrlechner MP;Qu L;McCoy JA;Murphy P;Hauck C;Maury W
薄荷科(Lamiaceae)产生多种具有药用价值的成分。已报道的几个家族成员具有抗病毒活性，包括柠檬香油(Melissa officinalis L.)、鼠尾草(Salvia spp.)、薄荷(Mentha×piperita L.)、牛膝草(Hyssopus officinalis L.)、罗勒(Ocimum spp.)和自愈(夏枯草属)。为了进一步研究夏枯草的抗慢病毒活性，我们测试了夏枯草水提物和乙醇提取物对HIV-1感染的抑制作用。与乙醇提取物相比，水提物含有更多的抗病毒活性，当浓度低于μg/mL时，对艾滋病毒-1显示出强大的抗病毒活性，而当浓度高出100倍以上时，几乎没有细胞毒性。添加时间研究表明，在感染开始后的前五个小时内加入水提取物是有效的，这表明植物成分针对的是进入事件。进一步分析表明，提取物既能抑制病毒/细胞相互作用，又能抑制结合后事件。虽然在我们的病毒/细胞相互作用研究中，最大限度地实现了40%的抑制，但提取物有效地阻止了结合后事件，其浓度与阻止感染的浓度相似，这表明针对后几步是介导抑制病毒感染性最重要的步骤。我们证明了普通平菇水提物抑制HIV-1的感染性。我们的研究表明，抑制主要通过早期、后病毒粒子结合事件的干扰发生。水提取物能够抑制艾滋病毒生命周期中的早期事件，这表明这些提取物或负责抗病毒活性的纯化成分是有希望的抗艾滋病毒-1的杀微生物剂和/或抗病毒药物。
The mint family (Lamiaceae) produces a wide variety of constituents with medicinal properties. Several family members have been reported to have antiviral activity, including lemon balm (Melissa officinalis L.), sage (Salvia spp.), peppermint (Mentha × piperita L.), hyssop (Hyssopus officinalis L.), basil (Ocimum spp.) and self-heal (Prunella vulgaris L.). To further characterize the anti-lentiviral activities of Prunella vulgaris, water and ethanol extracts were tested for their ability to inhibit HIV-1 infection. Aqueous extracts contained more anti-viral activity than did ethanol extracts, displaying potent antiviral activity against HIV-1 at sub μg/mL concentrations with little to no cellular cytotoxicity at concentrations more than 100-fold higher. Time-of-addition studies demonstrated that aqueous extracts were effective when added during the first five hours following initiation of infection, suggesting that the botanical constituents were targeting entry events. Further analysis revealed that extracts inhibited both virus/cell interactions and post-binding events. While only 40% inhibition was maximally achieved in our virus/cell interaction studies, extract effectively blocked post-binding events at concentrations similar to those that blocked infection, suggesting that it was targeting of these latter steps that was most important for mediating inhibition of virus infectivity. We demonstrate that aqueous P. vulgaris extracts inhibited HIV-1 infectivity. Our studies suggest that inhibition occurs primarily by interference of early, post-virion binding events. The ability of aqueous extracts to inhibit early events within the HIV life cycle suggests that these extracts, or purified constituents responsible for the antiviral activity, are promising microbicides and/or antivirals against HIV-1.