Cloning and isolation of human cytochrome P-450 cDNAs homologous to dioxin-inducible rabbit mRNAs encoding P-450 4 and P-450 6.
Cloning and isolation of human cytochrome P-450 cDNAs homologous to dioxin-inducible rabbit mRNAs encoding P-450 4 and P-450 6.
复制标题
与编码 P-450 4 和 P-450 6 的二恶英诱导兔 mRNA 同源的人细胞色素 P-450 cDNA 的克隆和分离。
DOI：
10.1089/dna.1985.4.395
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Tukey,RH
中科院分区：
文献类型：
--
作者：
Quattrochi,LC;Okino,ST;Pendurthi,UR;Tukey,RH
从人肝cDNA文库中克隆了与2，3，7，8-四氯-对-二恶英（TCDD）诱导的兔P-450 4和6结构相关的人细胞色素P-450。通过与兔P-450 4和P-450 6 cDNA的杂交，鉴定了人P-450 4 cDNA克隆hpP-450 4和人P-450 6 cDNA克隆hpP-450 6。DNA序列分析表明hpP-450 4与兔P-450 4和P-450 6的同源性分别为83%和75%，hpP-450 6与兔P-450 6和P-450 4的同源性分别为79%和72%。DNA序列比较表明，这两个人cDNA克隆的同源性为80%。这类似于在兔P-450 4和P-450 6的cDNA序列之间发现的同源性。北方印迹分析表明，人P-450 4 mRNA的长度约为3000个碱基，而人P-450 6 mRNA的长度为2600个碱基。克隆hpP-450 4优先与TCDD诱导的兔P-450 4和小鼠P3-450 mRNA杂交，而hpP-450 6优先与TCDD诱导的兔P-450 6和小鼠P1-450 mRNA杂交。hpP-450 4和hpP-450 6识别不同的基因组片段，表明它们各自由不同的基因编码。这些结果表明，人类中至少存在两个P-450基因，它们与兔和小鼠中TCDD诱导的P-450高度同源。
Human cytochrome P-450s structurally related to 2,3,7,8-tetrachloro-p-dioxin (TCDD)-inducible rabbit P-450 4 and 6 have been cloned from a human liver cDNA library. The human P-450 4 cDNA clone, hpP-450 4, and the human P-450 6 cDNA clone, hpP-450 6, were identified by hybridization to rabbit P-450 4 and P-450 6 cDNAs, respectively. DNA sequence analysis demonstrates that hpP-450 4 is 83% and 75% homologous to rabbit P-450 4 and P-450 6 mRNAs, respectively, whereas hpP-450 6 is 79% and 72% homologous to rabbit P-450 6 and P-450 4, respectively. A comparison of DNA sequence of the two human cDNA clones shows they are 80% homologous. This is similar to the homology found between the cDNA sequences of rabbit P-450 4 and P-450 6. Northern blot analysis has shown that the human P-450 4 mRNA is approximately 3000 bases, while the human P-450 6 mRNA is 2600 bases in length. Clone hpP-450 4 preferentially hybridizes to TCDD-inducible rabbit P-450 4 and mouse P3-450 mRNAs, whereas hpP-450 6 preferentially hybridizes to TCDD-inducible rabbit P-450 6 and mouse P1-450 mRNAs. Both hpP-450 4 and hpP-450 6 recognize different genomic fragments, indicating that each is encoded by different genes. These results indicate the existence of at least two P-450 genes in humans that are highly homologous to the TCDD-inducible P-450s in rabbits and mice.