Fast near-whole-brain imaging in adult Drosophila during responses to stimuli and behavior
Fast near-whole-brain imaging in adult Drosophila during responses to stimuli and behavior
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DOI：
10.1371/journal.pbio.2006732
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发表时间：
2019-02-01
期刊：
影响因子：
9.8
通讯作者：
Greenspan, Ralph J.
中科院分区：
文献类型：
--
作者：
Aimon, Sophie;Katsuki, Takeo;Greenspan, Ralph J.
全脑记录给了我们一个大脑活动的全局视角。在这项研究中，我们描述了一种使用光场显微镜记录行为的成年果蝇在高速下近全脑钙和电压活动的方法。我们首先获得了各种刺激和行为的全球活动图。值得注意的是，我们发现，当苍蝇走路时，大脑活动在全球范围内都有所增加，但在梳理时却没有。行走时多巴胺神经元的整体增加尤为明显。其次，我们使用主成分分析和独立成分分析提取了空间上不同的活动来源及其时间序列的地图。图中的特征形状与亚神经区域的解剖结构相匹配，在某些情况下，与特定的神经元类型相匹配。大脑结构对光和气味的反应与之前的报告一致，证实了新技术的有效性。我们还观察到先前未表征的行为相关活动以及自发电压活动的模式。
Whole-brain recordings give us a global perspective of the brain in action. In this study, we describe a method using light field microscopy to record near-whole brain calcium and voltage activity at high speed in behaving adult flies. We first obtained global activity maps for various stimuli and behaviors. Notably, we found that brain activity increased on a global scale when the fly walked but not when it groomed. This global increase with walking was particularly strong in dopamine neurons. Second, we extracted maps of spatially distinct sources of activity as well as their time series using principal component analysis and independent component analysis. The characteristic shapes in the maps matched the anatomy of subneuropil regions and, in some cases, a specific neuron type. Brain structures that responded to light and odor were consistent with previous reports, confirming the new technique's validity. We also observed previously uncharacterized behavior-related activity as well as patterns of spontaneous voltage activity.