THE RIBOSOMAL INTERGENIC SPACER REGION - A TARGET FOR THE PCR BASED DIAGNOSIS OF TUBERCULOSIS
THE RIBOSOMAL INTERGENIC SPACER REGION - A TARGET FOR THE PCR BASED DIAGNOSIS OF TUBERCULOSIS
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DOI：
10.1016/0962-8479(94)90081-7
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发表时间：
1994-10-01
期刊：
影响因子：
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通讯作者：
GANNON, F
中科院分区：
文献类型：
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作者：
GLENNON, M;SMITH, T;GANNON, F
设定：目的：研究开发一种区分结核分枝杆菌复合群成员的DNA探针的可能性，并建立一种检测结核分枝杆菌复合群成员的方法。设计：来自结核分枝杆菌成员的核糖体基因间间隔区。结果：该区域100%的序列同源性排除了开发区分结核分枝杆菌复合群成员的探针的可能性。肺结核综合征 然而，有可能开发出一种DNA探针，可以特异性地检测M的成员。结论：建立了检测结核分枝杆菌复合群的特异性DNA探针。结核复合体及其在M.痰中结核检出率为100%。
Setting: DNA Diagnostics, The National Diagnostics Centre and University College Hospital, Galway, Republic of Ireland.Objective: To investigate the possibility of developing a DNA probe to distinguish the members of the Mycobacterium tuberculosis complex and develop an assay for the detection of M. tuberculosis in sputum.Design: The ribosomal intergenic spacer regions from the members of the M. tuberculosis complex were sequenced and analysed for the ability of this region to provide sequence diversity for DNA probe development.Results: The 100% sequence homology in this region precluded the development of a probe to distinguish the individual members of the M. tuberculosis complex. However it was possible to develop a DNA probe that could specifically detect only the members of the M. tuberculosis complex.Conclusion: A specific DNA probe was developed for the detection of the M. tuberculosis complex and its application to M. tuberculosis detection in sputum was demonstrated.