Metabolic Dependence of Green Tea on Plucking Positions Revisited: A Metabolomic Study
Metabolic Dependence of Green Tea on Plucking Positions Revisited: A Metabolomic Study
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DOI：
10.1021/jf202304z
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发表时间：
2011-10-12
影响因子：
6.1
通讯作者：
Hong, Young-Shick
中科院分区：
文献类型：
--
作者：
Lee, Jang-Eun;Lee, Bum-Jin;Hong, Young-Shick
利用核磁共振氢谱分析技术结合多元统计数据，研究了绿色茶整体代谢物组与采摘部位的关系。采用主成分分析（PCA）和正交投影潜在结构判别分析（OPLS-DA）等模式识别方法，对不同部位采摘的绿色茶鲜叶进行代谢区分。除了在绿色茶叶之间的明确代谢区分之外，随着绿色茶树的生长，观察到茶氨酸、咖啡因和没食子酸水平的升高，但儿茶素（例如表儿茶素（EC）、表没食子儿茶素（EGC）、表儿茶素-3-没食子酸酯（ECG）和表没食子儿茶素-3-没食子酸酯（EGCG））、葡萄糖和蔗糖水平的降低。另一方面，绿色茶叶越年轻，茶氨酸、咖啡因和没食子酸越多，但绿色茶叶中的儿茶素越少，揭示了茶氨酸和儿茶素水平之间的反向关联，这是由于随着绿色茶树的生长，茶氨酸被分解成儿茶素。此外，与茶叶相比，在绿色茶茎中观察到显著高水平的茶氨酸和低水平的儿茶素，这表明茶叶和茎之间存在明显的茶树代谢。这种代谢组学方法强调了解绿色茶叶对采摘位置的整体代谢依赖性，从而提供具有特定茶叶品质的绿色茶生产的独特信息。
The dependence of global green tea metabolome on plucking positions was investigated through H-1 nuclear magnetic resonance (NMR) analysis coupled with multivariate statistical data set. Pattern recognition methods, such as principal component analysis (PCA) and orthogonal projection on latent structure-discriminant analysis (OPLS-DA), were employed for a finding metabolic discrimination among fresh green tea leaves plucked at different positions from young to old leaves. In addition to clear metabolic discrimination among green tea leaves, elevations in theanine, caffeine, and gallic acid levels but reductions in catechins, such as epicatechin (EC), epigallocatechin (EGC), epicatechin-3-gallate (ECG), and epigallocatechin-3-gallate (EGCG), glucose, and sucrose levels were observed, as the green tea plant grows up. On the other hand, the younger the green tea leaf is, the more theanine, caffeine, and gallic acid but the lesser catechins accumlated in the green tea leaf, revealing a reverse assocation between theanine and catechins levels due to incorporaton of theanine into catechins with growing up green tea plant. Moreover, as compared to the tea leaf, the observation of marked high levels of theanine and low levels of catechins in green tea stems exhibited a distinct tea plant metabolism between the tea leaf and the stem. This metabolomic approach highlights taking insight to global metabolic dependence of green tea leaf on plucking position, thereby providing distinct information on green tea production with specific tea quality.