Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the beta-glucuronidase gene in transgenic tomato plants
Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the beta-glucuronidase gene in transgenic tomato plants
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DOI：
10.1007/s004250050207
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发表时间：
1997-12-01
期刊：
影响因子：
4.3
通讯作者：
Ryan, CA
中科院分区：
文献类型：
--
作者：
Jacinto, T;McGurl, B;Ryan, CA
番茄（Lycopersicon esculentum Mill.）CV. Better Boy）植物用含有番茄前系统素基因的2.2-kb启动子片段和β-葡萄糖醛酸酶（GUS）报告基因的编码区的融合基因转化。通过组织化学染色和GUS酶活性测定，转基因植株表现出低组成水平的前系统素-β-葡萄糖醛酸苷酶基因表达，其与叶主脉、小叶柄、叶柄和茎的维管束相关。在每个组织中的维管束中的GUS活性增加的创伤和茉莉酸甲酯，类似于在野生型植物中的原系统素的诱导植物的治疗。GUS活性的增加，在维管束的叶片在响应创伤相关的创伤诱导的增加prosystemin mRNA。组织打印，使用兔抗血清制备的抗prosystemin，证实了诱导prosystemin蛋白定位于维管束的小叶柄，叶柄和茎的野生型番茄植物。有证据表明，番茄prosystemin基因的2.2-kb启动子区含有赋予其在转基因番茄植物的维管束中正确的时空表达的元件。
Tomato (Lycopersicon esculentum Mill. cv. Better Boy) plants were transformed with a fused gene containing a 2.2-kb promoter fragment of the tomato prosystemin gene and the coding region of the beta-glucuronidase (GUS) reporter gene. The transgenic plants exhibited a low constitutive level of prosystemin-beta-glucuronidase gene expression, assayed by histochemical staining and GUS enzyme activity, that was associated in the vascular bundles of leaf main veins, petiolules, petioles and stems. The GUS activity in the vascular bundles in each tissue was increased by wounding and by treatment of the plants with methyl jasmonate, similar to the induction of prosystemin in wild-type plants. The increase in GUS activity in the vascular bundles of leaves in response to wounding correlated with the wound-inducible increase in prosystemin mRNA. Tissue printing, using rabbit anti-serum prepared against prosystemin, confirmed that inducible prosystemin protein was localized in vascular bundles of petiolules, petioles and stems of wild-type tomato plants. The evidence indicates that the 2.2-kb promoter region of the tomato prosystemin gene contains elements conferring its correct temporal and spatial expression in the vascular bundles of transgenic tomato plants.