CHEMICAL MODIFICATION OF PULULAN .1. PERIODATE-OXIDATION
CHEMICAL MODIFICATION OF PULULAN .1. PERIODATE-OXIDATION
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DOI：
10.1016/0032-3861(93)90600-f
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发表时间：
1993-01-01
期刊：
影响因子：
4.6
通讯作者：
SCHACHT, E
中科院分区：
文献类型：
--
作者：
BRUNEEL, D;SCHACHT, E
本文研究了高碘酸氧化普鲁兰多糖的方法。特别关注的是活化产物的结构和特性。普鲁兰多糖在重复单元中含有三个不同的脱水葡糖苷部分。因此，普鲁兰的高碘酒氧化会产生不同类型的二醛结构。通过滴定分析普鲁兰多糖氧化过程中产生的甲酸，可以计算出单氧化和双氧化脱水葡萄糖苷单元的数量。通过与盐酸羟胺反应测定的完全高碘酸盐氧化的普鲁兰中的醛含量仅为67%。一个合理的解释是存在一个稳定的六元半缩醛。还原实验收集的数据和C-13 n.m.r.也证明了具有不同反应性的醛基的存在。最后，研究了多糖骨架的水解稳定性。
The present paper describes the periodate oxidation of pullulan. Special attention is paid to the structures and characteristics of the activated products. Pullulan contains three different anhydroglucoside moieties in the repeating unit. Therefore, periodate oxidation of pullulan results in different types of dialdehyde structures. From titrimetric analysis of the formic acid generated during the oxidation of pullulan, the numbers of singly and doubly oxidized anhydroglucoside units can be calculated. The aldehyde content in completely periodate-oxidized pullulan as determined by reaction with hydroxylamine hydrochloride is only 67%. A plausible explanation is the presence of a stable, six-membered hemiacetal. The presence of aldehyde groups with different reactivities is also evidenced by the data collected from reduction experiments and by C-13 n.m.r. Finally, the hydrolytic stability of the polysaccharide backbone was studied.