Lipid rafts of purified mouse brain synaptosomes prepared with or without detergent reveal different lipid and protein domains
Lipid rafts of purified mouse brain synaptosomes prepared with or without detergent reveal different lipid and protein domains
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DOI：
10.1016/s0006-8993(02)03986-0
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发表时间：
2003-02-07
期刊：
影响因子：
2.9
通讯作者：
Wood, WG
中科院分区：
文献类型：
--
作者：
Eckert, GP;Igbavboa, U;Wood, WG
脂筏在脂质转运、信号转导和细胞生长等方面发挥着重要作用。越来越多的证据表明，脂筏可能在脑细胞功能中发挥作用。脂筏通常使用去污剂如Triton X-100分离。然而，来自非脑组织的数据表明，与非去污剂制备相比，使用去污剂制备脂筏可能代表不同的筏结构域。本研究的目的是使用高度纯化的小鼠突触体组分以及非去污剂和去污剂方法比较脂筏的蛋白质和脂质标记物。脂筏标记蛋白，碱性磷酸酶和flotillin，和脂质标记，胆固醇和鞘磷脂，是高度富集的脂筏制备与洗涤剂相比，非洗涤剂馏分。Na+，K+-ATP酶的富集是更大的非洗涤剂的脂筏组分相比，与洗涤剂制备的脂筏。用或不用去污剂制备的来自神经元的神经末梢的脂筏可能代表不同的膜结构域，每个膜结构域具有独特的专门功能。(C)2002 Elsevier Science B. V.保留所有权利。
Lipid rafts have been proposed to be important in a variety of functions including lipid transport, signal transduction and cell growth. There is increasing evidence that lipid rafts may play a role in cell functions in brain. Lipid rafts are typically isolated using a detergent such as Triton X-100. There has been, however, data from non-brain tissue indicating that preparation of lipid rafts using a detergent may represent different raft domains as compared with non-detergent preparation. The purpose of the present study was to compare protein and lipid markers of lipid rafts using a highly purified mouse synaptosomal fraction and non-detergent and detergent methods. The lipid raft marker proteins, alkaline phosphatase and flotillin, and the lipid markers, cholesterol and sphingomyelin, were highly enriched in lipid rafts prepared with detergent as compared with the non-detergent fraction. Enrichment of Na+,K+-ATPase was greater in the non-detergent lipid raft fraction as compared with lipid rafts prepared with detergent. Lipid rafts from the nerve terminal of neurons prepared with or without detergents may represent different membrane domains each with unique specialized functions. (C) 2002 Elsevier Science B.V. All rights reserved.