Specific adsorption of IgM antibody onto H-2-activated mouse T lymphocytes.
Specific adsorption of IgM antibody onto H-2-activated mouse T lymphocytes.
复制标题
IgM抗体在H-2激活的小鼠T淋巴细胞上的特异性吸附。
DOI：
10.1084/jem.143.2.444
复制
发表时间：
1976-02-01
影响因子：
15.3
通讯作者：
Sprent, J
中科院分区：
文献类型：
--
作者：
Hudson, L;Sprent, J
有证据支持这样的观点，即H-2激活的T细胞表面免疫荧光染色显示的IgM代表特异性吸附的B细胞来源的同种抗体。被H-2决定簇激活的T细胞只有在祖细胞群中含有B淋巴细胞时才表达表面IgM。在被不能刺激同种异体抗体产生的决定因素(例如，M基因决定因素)激活的T细胞上未检测到IGM。此外，与M位点激活的T细胞不同，IgM阴性的H-2激活的T细胞(来源于B细胞耗尽的淋巴样细胞)在体外与针对激活的H-2决定簇而产生的“早期出血”抗血清孵育时，以一种特定的方式吸附IgM。
Evidence is presented to support the contention that IgM demonstrable by surface immunofluorescent staining on H-2-activated T cells represents specifically adsorbed B-cell-derived alloantibody. T cells activated to H-2 determinants expressed surface IgM only when the progenitor cell populations contained B lymphocytes. IgM was not detected on T cells activated to determinants which fail to stimulate alloantibody production (e.g., M-locus determinants). In addition, IgM- negative H-2 activated T cells (derived from B-cell-depleted lymphoid cells), unlike M-locus-activated T cells, adsorbed IgM in a specific manner when incubated in vitro with "early bleed" antisera raised against the activating H-2 determinants.