Relationship of infectious murine leukemia virus and virus-related antigens in genetic crosses between AKR and the Fv-1 compatible strain C57L.
Relationship of infectious murine leukemia virus and virus-related antigens in genetic crosses between AKR and the Fv-1 compatible strain C57L.
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DOI：
10.1084/jem.143.1.32
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
Jacobs S
中科院分区：
文献类型：
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作者：
Ikeda H;Rowe WP;Boyse EA;Stockert E;Sato H;Jacobs S
在对小鼠白血病病毒（MuLV）及其组分的进一步遗传研究中，我们检查了回交C57 LX（C57 LX AKR）。选择该群体是因为AKR和C57 L株都是Fv-1 n，并且因此消除了Fu-1b等位基因对病毒输出的限制。对分离物的三个特征进行评分：（a）尾部的感染性Gross-AKR型MuLV（V）;（B）脾脏中指示p30内部病毒蛋白的组特异性抗原;和（c）胸腺细胞上的GIX抗原，现在认为是gp 69/71病毒包膜糖蛋白的指示。我们的结论是：（a）确认AKR小鼠具有两个非连锁的染色体基因Akv- 1和Akv-2，每个基因都可以独立地产生AKR小鼠特有的MuLV终身高输出。(b)在可能来自三对独立的替代性状分离的八种表型中，观察到七种，但在后代测试中，只有三种表现出稳定遗传的基因型;这些是V+p30+GIX+和V-p30-GIX-（亲本类型）和V-p30+GIX+。第三个新鉴定的AKR基因，命名为Akvp，独立于Akv-1和Akv-2分离，也决定p30和GIX的表达，但在这种情况下独立于XC-可检测的MuLV。(c)其余四个观察到的表型，没有繁殖后代测试，主要涉及抗原阴性的父母产生抗原阳性的后代，这是可能的，这些差异表示抑制表型的母体抗性因子。
In a further genetic study of murine leukemia virus (MuLV) and its components we examined the backcross C57L X (C57L X AKR). This population was selected because strains AKR and C57L are both Fv-1n, and the restriction which the Fu-1b allele imposes on the output of virus was thereby obviated. The segregants were scored for three characters: (a) infectious Gross-AKR-type MuLV (V), in the tail; (b) group-specific antigen indicative of p30 internal viral protein, in spleen; and (c) GIX antigen, now thought to be indicative of gp69/71 viral envelope glycoprotein, on thymocytes. Our conclusions are: (a) It is confirmed that the AKR mouse has two unlinked chromosomal genes, Akv- 1 and Akv-2, each of which can independently give rise to the life-long high output of MuLV that is characteristic of AKR mice. (b) Of the eight phenotypes that could possibly be derived from segregation of the three pairs of independent alternative traits, seven were observed, but on progeny testing only three were shown to reflect stably heritable genotypes; these were V+p30+GIX+ and V-p30-GIX- (the parental types) and V-p30+GIX+. A third, newly identified AKR gene, designated Akvp, segregating independently of Akv-1 and Akv-2, also determines expression of p30 and GIX but in this case independently of XC- detectable MuLV. (c) The four remaining observed phenotypes, which did not breed true on progeny testing, involved mostly antigen-negative parents yielding antigen-positive progeny; it is likely that these discrepancies represented suppression of phenotype by a maternal resistance factor.