Development of a Glycoform Library-based Strategy to Decipher the Role of Protein Glycosylation.
Development of a Glycoform Library-based Strategy to Decipher the Role of Protein Glycosylation.
复制标题
开发基于糖型库的策略来破译蛋白质糖基化的作用。
DOI：
10.1007/978-1-0716-2489-0_14
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Tan,Zhongping
中科院分区：
文献类型：
--
作者：
Li,Yaohao;Ma,Bo;Li,Xue;Shang,Shiying;Tan,Zhongping
从细胞培养上清液或裂解物中获得的糖蛋白通常以超过100种不同的糖基化蛋白形式（糖形式）的混合物存在。由于糖蛋白的异质性，糖基化的研究受到了很大的阻碍。为了克服这一挑战，我们开发并优化了一种基于糖型库的策略来研究蛋白质糖基化的作用。在该策略中，采用化学合成方法制备单个均匀糖型，并通过比较一系列糖基化模式存在系统差异的糖型来确定糖基化的作用。
Glycoproteins obtained from cell culture supernatants or lysates generally exist as mixtures of over 100 differently glycosylated protein forms (glycoforms). The study of glycosylation is significantly impeded because of the heterogeneous nature of glycoproteins. To overcome this challenge, we developed and optimized a glycoform library-based strategy to investigate the role of protein glycosylation. In this strategy, chemical synthesis was used to prepare individual homogeneous glycoforms and the role of glycosylation was determined by comparing a series of glycoforms with systematic differences in their glycosylation patterns.