Leishmania mexicana and Leishmania major:: Attenuation of wild-type parasites and vaccination with the attenuated lines
Leishmania mexicana and Leishmania major:: Attenuation of wild-type parasites and vaccination with the attenuated lines
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DOI：
10.1086/374783
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发表时间：
2003-05-15
影响因子：
6.4
通讯作者：
Phillips, RS
中科院分区：
文献类型：
--
作者：
Daneshvar, H;Coombs, GH;Phillips, RS
通过在庆大霉素压力下体外培养减毒利什曼原虫的方法已成功地用于墨西哥利什曼原虫，L。梅杰湖infantum和L. donovani。减毒系侵入但不能在体外骨髓源性巨噬细胞内存活，而野生型寄生虫存活并繁殖。L. mexicana和L. major在皮下注射稳定期寄生虫后，在大多数BALB/c小鼠中，在至少12周的观察期内均未能诱导皮肤病变。L.在40次传代培养中，墨西哥在无庆大霉素的培养基中保留了其特性。L. mexicana和L. Major均诱导小鼠对野生型寄生虫攻击的显著保护。
A method for attenuation of Leishmania species by culturing in vitro under gentamicin pressure has been used successfully with Leishmania mexicana, L. major, L. infantum, and L. donovani. The attenuated lines invaded but were unable to survive within bone marrow-derived macrophages in vitro, whereas wild-type parasites survived and multiplied. The attenuated lines of L. mexicana and L. major both failed to induce cutaneous lesions in the majority of BALB/c mice over a minimum 12-week observation period after subcutaneous injection of stationary phase parasites. The attenuated line of L. mexicana retained its properties in gentamicin-free medium over 40 subcultures. The attenuated lines of L. mexicana and L. major both induced significant protection in mice against challenge with wild-type parasites.