EFFECTS OF RETINOIC ACID (RA) ON THE GROWTH AND PHENOTYPIC-EXPRESSION OF SEVERAL HUMAN NEURO-BLASTOMA CELL-LINES
EFFECTS OF RETINOIC ACID (RA) ON THE GROWTH AND PHENOTYPIC-EXPRESSION OF SEVERAL HUMAN NEURO-BLASTOMA CELL-LINES
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DOI：
10.1016/0014-4827(83)90184-2
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发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
SEEGER, RC
中科院分区：
文献类型：
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作者：
SIDELL, N;ALTMAN, A;SEEGER, RC
维甲酸（RA）能促进人神经母细胞瘤细胞系LA-N-1的形态分化并抑制其生长。本研究通过确定RA对其他7种人神经母细胞瘤细胞系的影响来测试这些现象的组织学一般性。RA强烈抑制锚定依赖性生长，并诱导7个细胞系中的6个的形态学改变。这些变化包括形态分化，如在4个细胞系中形成神经突延伸，在1个培养物中细胞增大和空泡化，以及在另一个培养物中形成大的扁平上皮或成纤维细胞样细胞。尽管在单层培养中有1个细胞系对RA的作用相对不敏感，但在软琼脂试验中，所有7个细胞系都被RA强烈抑制。在2/2的检测系中检测到细胞RA结合蛋白。显然，作为一个组织学组，人神经母细胞瘤细胞是非常敏感的RA诱导的生长抑制和形态学改变通常与这种类型的癌症的恶性表型的表达减少。
Retinoic acid (RA) can promote morphologic differentiation and inhibit the growth of a human neuroblastoma cell line, LA-N-1. This study tests the histological generality of these phenomena by determining the effects of RA on 7 other human neuroblastoma cell lines. RA strongly inhibited anchorage-dependent growth and induced morphologic alterations in 6 of 7 cell lines. These alterations included morphologic differentiation as evidenced by formation of neurite extensions in 4 of the lines, cellular enlargement and vacuolization in 1 culture and formation of large, flattened epithelial or fibroblastic like cells in another culture. Although 1 cell line was relatively insensitive to the effects of RA in monolayer culture, all 7 were strongly inhibited by RA in soft agar assays. Cellular RA-binding proteins were detected in 2/2 lines tested. Apparently, as a histological group, human neuroblastoma cells are extremely sensitive to RA-induced growth inhibition and morphological alterations generally associated with reduced expression of the malignant phenotype of this type of cancer.