Effective Sample Preparations in Imaging Mass Spectrometry.
Effective Sample Preparations in Imaging Mass Spectrometry.
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DOI：
10.5702/massspectrometry.s0029
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Sugiura, Yuki
中科院分区：
文献类型：
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作者：
Shimma, Shuichi;Sugiura, Yuki
基质辅助激光解吸/电离（MALDI）成像质谱（IMS）可用于可视化生物分子（蛋白质、肽、代谢物）和药物在组织表面上的分布。在MALDI-IMS中，样品制备对于成功的结果至关重要。各种条件，如组织取样方法、组织厚度和基质应用程序可能对结果产生影响。在这篇综述中，我们总结了每个样品制备步骤的顺序与实际的例子。此外，我们讨论了基质溶液中使用的有机溶剂的重要性。基质溶液中使用的有机溶剂的组成对于在该方法中实现高灵敏度至关重要。
Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) can be used to visualize the distribution of biomolecules (proteins, peptides, metabolites) and drugs on tissue surfaces. In MALDI-IMS, sample preparation is crucial for successful results. A variety of conditions, such as tissue sampling methods, tissue thickness and matrix application procedure can have an impact on the results. In this review, we summarize each sample preparation step in an orderly sequence with practical examples. In addition, we discuss the importance of the organic solvent used in the matrix solution. The composition of the organic solvent used in the matrix solution is critical for achieving a high sensitivity in this procedure.