In vitro negative selection of viral superantigen-reactive thymocytes by thymic dendritic cells
In vitro negative selection of viral superantigen-reactive thymocytes by thymic dendritic cells
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DOI：
10.1182/blood.v90.5.1943
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发表时间：
1997-09-01
期刊：
影响因子：
20.3
通讯作者：
Ardavin, C
中科院分区：
文献类型：
--
作者：
Ferrero, I;Anjuere, F;Ardavin, C
内源性小鼠乳腺肿瘤病毒 (MMTV) 编码的超抗原 (SAg) 的胸腺内表达会诱导带有 SAg 反应性 T 细胞受体 (TCR) V beta 元件的 T 细胞的克隆删除。然而，参与诱导 SAg 耐受的胸腺抗原呈递细胞 (APC) 的身份仍有待确定。我们在体外测定中分析了树突状细胞 (DC) 介导 Mtv-7 反应性 TCR αβ P14 转基因胸腺细胞克隆缺失的潜力。我们的结果表明，胸腺和脾脏 DC 均诱导 TCR 转基因双阳性 (DP) 胸腺细胞的缺失，在该实验系统中，DC 似乎比脾 B 细胞更有效地负选择 APC。有趣的是，胸腺和脾DC表现出不同的诱导CD4(+)SP胸腺细胞增殖的能力。这些观察结果表明胸腺 DC 可能在体内诱导 SAg 耐受性中发挥重要作用。 (C) 1997 年，美国血液学会。
Intrathymic expression of endogenous mouse mammary tumor virus (MMTV)-encoded superantigens (SAg) induces the clonal deletion of T cells bearing SAg-reactive T-cell receptor (TCR) V beta elements. However, the identity of the thymic antigen-presenting cells (APC) involved in the induction of SAg tolerance remains to be defined, We have analyzed the potential of dendritic cells (DC) to mediate the clonal deletion of Mtv-7-reactive TCR alpha beta P14 transgenic thymocytes in an in vitro assay. Our results show that both thymic and splenic DC induced the deletion of TCR transgenic double positive (DP) thymocytes, DC appear to be more efficient than splenic B cells as negatively selecting APC in this experimental system. Interestingly, thymic and splenic DC display a differential ability to induce CD4(+)SP thymocyte proliferation. These observations suggest that thymic DC may have an important role in the induction of SAg tolerance in vivo. (C) 1997 by The American Society of Hematology.