The first successful observation of in-cell NMR signals of DNA and RNA in living human cells
The first successful observation of in-cell NMR signals of DNA and RNA in living human cells
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首次成功观察活体人类细胞中 DNA 和 RNA 的细胞内 NMR 信号
DOI：
10.1039/c7cp05188c
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发表时间：
2018
影响因子：
3.3
通讯作者：
Katahira Masato
中科院分区：
文献类型：
--
作者：
Yamaoki Yudai;Kiyoishi Ayaka;Miyake Masayuki;Kano Fumi;Murata Masayuki;Nagata Takashi;Katahira Masato
为了了解细胞内的生物学事件，有关活细胞中蛋白质和核酸的结构、动力学和相互作用的信息至关重要。细胞内NMR是一种很有前途的方法来获得这些信息。尽管已经报道了人类细胞中蛋白质的NMR信号，但仅在非洲爪蟾卵母细胞中报道了核酸的NMR信号，即，而不是人类细胞。在这里，DNA和RNA通过细菌毒素链球菌溶血素O的孔形成和随后的重新密封被引入人类细胞。然后，在活体人类细胞中首次成功观察到DNA和RNA的NMR信号。观察到的信号直接表明在活的人类细胞中形成了DNA和RNA发夹结构。
In order to understand intracellular biological events, information on the structure, dynamics and interaction of proteins and nucleic acids in living cells is of crucial importance. In-cell NMR is a promising method to obtain this information. Although NMR signals of proteins in human cells have been reported, those of nucleic acids were reported only in Xenopus laevis oocytes, i.e., not in human cells. Here, DNA and RNA were introduced into human cells by means of pore formation by bacterial toxin streptolysin O and subsequent resealing. Then, NMR signals of DNA and RNA were successfully observed for the first time in living human cells. The observed signals directly suggested the formation of DNA and RNA hairpin structures in living human cells.