On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis
On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis
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DOI：
10.1023/a:1026228518418
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发表时间：
2003-11
影响因子：
2.7
通讯作者：
A. Surribas;O. Cós;J. Montesinos;F. Valero
中科院分区：
文献类型：
--
作者：
A. Surribas;O. Cós;J. Montesinos;F. Valero
利用乙醇氧化酶和过氧化物酶的酶促反应，建立了一种基于停流技术的自动顺序注射分析（SIA）系统。它的应用作为一个在线监测装置pastorisPichia生产异源蛋白的发酵进行了演示。通过在SIA歧管中包括稀释室，可以达到线性响应，观察到高达2 g l−1。采样频率为每小时7次，相对标准偏差小于4%。
An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoringPichia pastorisfermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l−1, was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.