Arsenic biotransformation by arsenic-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1.
Arsenic biotransformation by arsenic-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1.
复制标题
DOI：
10.1016/j.scitotenv.2011.08.039
复制
发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
S. Su;X. Zeng;L. Bai;Lianfang Li;R. Duan
中科院分区：
文献类型：
--
作者：
S. Su;X. Zeng;L. Bai;Lianfang Li;R. Duan
利用微生物对砷（As）污染土壤进行生物修复一直是研究的热点，因为它环境友好且具有成本效益。将抗砷真菌Trichoderma asperellum SM-12F1、Penicillium janthinellum SM-12F4和Fusarium oxysporum CZ-8F1暴露于50mgl−1的As(V)中，研究了As的生物转化以及培养基中Eh和pH的变化。所有三个分离株的鲜重随培养时间的不同而先增加后减少。培养2或3天后，添加到培养基中的As(V)已完全转变为As(III)，而培养过程中真菌细胞中以As(V)为主，同时伴随少量As(III)。 15天后，根据培养物中As形态定量分析，T. asperellum SM-12F1细胞中除As(V)和As(III)外，还发现少量的一甲基胂酸(MMA)和二甲基胂酸(DMA)，其中总As含量以P. janthinellum SM-12F4细胞最高(约41.5μg)。此外，当培养时间达到3天时，T. asperellum SM-12F1（312.5mV和4.8）、P. janthinellum SM-12F4（411.1mV和4.2）和F. oxysporum CZ-8F1（269.4mV和4.8）培养基中的Eh和pH可能不会导致As（V）的减少。以前的研究。因此，推测As的输入/输出、还原和甲基化是在真菌细胞中进行的。未来需要研究真菌对砷的生化行为，以更好地了解砷污染土壤的生物修复。
Bioremediation of arsenic (As)-contaminated soil using microorganisms has been a focus of research because it is environment friendly and cost-effective. The As-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were exposed to 50mgl−1of As(V), and the biotransformation of As and the concomitant variance of Eh and pH in the media were studied. Fresh weights of all three isolates increased and then decreased depended on cultivation period. After cultivation for 2 or 3days, the As(V) added to the media had been completely changed into As(III), whilst As(V) was predominate in fungal cells with concomitantly little As(III) during cultivation. After 15days, little monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) besides of As(V) and As(III) were found in the cells of T. asperellum SM-12F1, and the total As content was the highest in cells of P. janthinellum SM-12F4 (about 41.5μg) according to the quantitative analysis of As speciation in cultures. Moreover, when cultivation period reached 3days, the Eh and pH in the media of T. asperellum SM-12F1 (312.5mV and 4.8), P. janthinellum SM-12F4 (411.1mV and 4.2), and F. oxysporum CZ-8F1 (269.4mV and 4.8) might not responsible for the reduction of As(V) based on the previous study. Therefore, it is speculated that import/export, reduction, and methylation of As are conducted in fungal cells. Future studies investigating the biochemical behaviour of fungi responding to As are needed to gain a better understanding of bioremediation of As-contaminated soils.