Additive effects of exogenous IL-12 supplementation and antibiotic treatment in infection prophylaxis.
Additive effects of exogenous IL-12 supplementation and antibiotic treatment in infection prophylaxis.
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DOI：
10.1002/jor.21520
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发表时间：
2012-02
影响因子：
2.8
通讯作者：
Li, Bingyun
中科院分区：
文献类型：
--
作者：
Boyce, Brandon M.;Lindsey, Brock A.;Clovis, Nina B.;Smith, E. Suzanne;Hobbs, Gerald R.;Hubbard, David F.;Emery, Sanford E.;Barnett, John B.;Li, Bingyun
开放性骨折相关感染的临床发病率和宿主风险的增加，以及传统抗生素治疗此类感染的有效性的降低，推动了预防开放性骨折相关感染的新疗法的发展。我们研究了在开放性骨折部位经皮补充一种天然细胞因子（即白细胞介素12p70或IL-12）以减少开放性骨折相关感染。我们还确定了IL-12联合常规抗生素治疗预防开放性骨折相关感染的疗效。采用大鼠股骨骨折后直接接种金黄色葡萄球菌临床分离物建立开放性股骨骨折感染模型。这些动物被分为四组：不给药，经皮补充IL-12，腹腔注射抗生素氨苄西林，或经皮注射IL-12与腹腔注射氨苄西林。分别于术后第6、10、14、21天实施安乐死。经皮IL-12导致术后第6天和第10天感染减少。首次发现外源性IL-12与常规治疗（即抗生素治疗）联合使用时在预防感染方面具有加性作用。氨苄西林和IL-12联合治疗显著降低了术后第6天的感染率，也缩短了完全抑制感染所需的时间。因此，外源性IL-12提供了一种独立于抗生素耐药性的保护机制，补充了抗生素的常规使用。
The increasing clinical incidence and host risk of open fracture-associated infections, as well as the reduced effectiveness of conventional antibiotics to treat such infections, have driven the development of new therapies for the prophylaxis of open fracture-associated infections. We investigated percutaneous supplementation of a natural cytokine (i.e. interleukin 12p70 or IL-12) at an open fracture site to reduce open fracture-associated infections. We also determined the efficacy of the combination therapy of IL-12 and conventional antibiotic therapy in the prophylaxis of open fracture-associated infections. An open femur fracture infection model was produced by direct inoculation of a clinical isolate of Staphylococcus aureus after creating a femur fracture using rats. The animals were assigned to one of four groups: no drug administration, percutaneous supplementation of IL-12, intraperitoneal administration of the antibiotic ampicillin, or percutaneous IL-12 in combination with intraperitoneal ampicillin. Animals were euthanized at post-operative days 6, 10, 14, and 21. Percutaneous IL-12 led to a reduction in infection at post-operative days 6 and 10. For the first time, exogenous IL-12 was found to have additive effects in the prevention of infection when combined with conventional treatment (i.e. antibiotic therapy). Combination therapy of ampicillin and IL-12 substantially reduced the infection rate at post-operative day 6 and also decreased the time needed for complete inhibition of infection. Therefore, exogenous IL-12, providing a mechanism of protection independent of antibiotic resistance, complements the routine use of antibiotics.
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影响因子：
4.1
作者：
Lucke, M;Wildemann, B;Schmidmaier, G
通讯作者：
Schmidmaier, G
影响因子：
3
作者：
Bonville CA;Percopo CM;Dyer KD;Gao J;Prussin C;Foster B;Rosenberg HF;Domachowske JB
通讯作者：
Domachowske JB
影响因子：
14
作者：
Li, Bingyun;Jiang, Bingbing;Boyce, Brandon M.;Lindsey, Brock A.
通讯作者：
Lindsey, Brock A.
影响因子：
3
作者：
Brune, IB;Wilke, W;Siewert, JR
通讯作者：
Siewert, JR
影响因子：
5.7
作者：
Flohe, Stefanie B.;Agrawal, Hemant;Schade, F. Ulrich
通讯作者：
Schade, F. Ulrich