Neuronal somatic plasmalemmal permeability and dendritic beading caused by head rotational traumatic brain injury in pigs-An exploratory study.
Neuronal somatic plasmalemmal permeability and dendritic beading caused by head rotational traumatic brain injury in pigs-An exploratory study.
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DOI：
10.3389/fncel.2023.1055455
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发表时间：
2023
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
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闭合性头部创伤性脑损伤(TBI)是由头部的快速运动引起的，导致整个大脑的弥漫应变场。损伤机制(S)、负荷阈值和受影响细胞的神经解剖学分布仍然知之甚少，特别是在脑回。我们利用猪模型来探讨快速头部旋转加减速负荷与大脑皮层、皮质下白质和海马区血浆通透性的即刻变化之间的关系。为了评估血浆膜损害，在伤后15分钟实施安乐死的动物在伤前脑室注射小细胞非故意染料荧光黄(LY)，并在实质内扩散；其他动物(未接受LY)存活到8小时或7天。质膜通透性多见于神经元胞体和树突，而白质轴突少见。基于头部旋转运动学，特别是最大角速度，LY+神经元的负荷增加，并因重复的颅脑损伤而加剧。在大脑皮层，LY+细胞在内侧回和外侧回均有明显表达。在海马区和内嗅区皮质内观察到神经细胞膜的通透性，包括树突中的珠状等形态变化。这些变化与海马体中随后时间点的纤维突起减少和突触电流变化有关。进一步的组织学观察发现，在LY+和LY-细胞中，NeuN免疫反应性降低，线粒体分裂增加，caspase途径激活，提示存在多种损伤表型。这项探索性研究表明，在闭合式旋转脑损伤中，神经元胞体的质膜破坏与皮质和海马灰质内的树突之间的关系是主要的反应，并为未来的传统假设检验实验奠定了基础。
Closed-head traumatic brain injury (TBI) is induced by rapid motion of the head, resulting in diffuse strain fields throughout the brain. The injury mechanism(s), loading thresholds, and neuroanatomical distribution of affected cells remain poorly understood, especially in the gyrencephalic brain. We utilized a porcine model to explore the relationships between rapid head rotational acceleration-deceleration loading and immediate alterations in plasmalemmal permeability within cerebral cortex, sub-cortical white matter, and hippocampus. To assess plasmalemmal compromise, Lucifer yellow (LY), a small cell-impermeant dye, was delivered intraventricularly and diffused throughout the parenchyma prior to injury in animals euthanized at 15-min post-injury; other animals (not receiving LY) were survived to 8-h or 7-days. Plasmalemmal permeability preferentially occurred in neuronal somata and dendrites, but rarely in white matter axons. The burden of LY+ neurons increased based on head rotational kinematics, specifically maximum angular velocity, and was exacerbated by repeated TBI. In the cortex, LY+ cells were prominent in both the medial and lateral gyri. Neuronal membrane permeability was observed within the hippocampus and entorhinal cortex, including morphological changes such as beading in dendrites. These changes correlated with reduced fiber volleys and synaptic current alterations at later timepoints in the hippocampus. Further histological observations found decreased NeuN immunoreactivity, increased mitochondrial fission, and caspase pathway activation in both LY+ and LY– cells, suggesting the presence of multiple injury phenotypes. This exploratory study suggests relationships between plasmalemmal disruptions in neuronal somata and dendrites within cortical and hippocampal gray matter as a primary response in closed-head rotational TBI and sets the stage for future, traditional hypothesis-testing experiments.
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影响因子：
2.5
作者：
Chen, ZM;Kontonotas, D;Neumar, RW
通讯作者：
Neumar, RW
影响因子：
3.3
作者：
Davoli, MA;Fourtounis, J;Xu, D
通讯作者：
Xu, D
影响因子：
4.2
作者：
Geddes, DM;Cargill, RS;LaPlaca, MC
通讯作者：
LaPlaca, MC
DOI：
10.1115/1.2895464
发表时间：
1993-02-01
影响因子：
1.7
作者：
GALBRAITH, JA;THIBAULT, LE;MATTESON, DR
通讯作者：
MATTESON, DR
影响因子：
4.2
作者：
Friess, Stuart H.;Ichord, Rebecca N.;Margulies, Susan S.
通讯作者：
Margulies, Susan S.