Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells.
Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells.
复制标题
皮肤干细胞作为原始生殖细胞和卵母细胞样细胞的来源
DOI：
10.1038/cddis.2016.366
复制
发表时间：
2016-11-10
影响因子：
9
通讯作者：
Shen W
中科院分区：
文献类型：
--
作者：
Ge W;Cheng SF;Dyce PW;De Felici M;Shen W
皮肤是一个独特的器官，含有各种干细胞，用于维持皮肤稳态和修复损伤和疾病后的皮肤组织。皮肤源性干细胞（SDSC）是由真皮体外培养产生的一种异质性干细胞群，可在悬浮培养中以球形细胞聚集体的形式培养。在某些体外或体内条件下，SDSC显示出多能性，并且可以产生多种神经、中胚层和内胚层细胞类型，例如神经元、神经胶质、成纤维细胞、脂肪细胞、肌肉细胞、成软骨细胞、成骨细胞和胰岛β细胞样细胞。SDSC可能来源于位于毛囊中的多能干细胞，而毛囊又来源于胚胎迁移神经嵴或中胚层细胞。在过去的十年中，一波报告表明，生殖细胞可以从各种类型的干细胞中产生。已经显示SDSC能够在体外产生原始生殖细胞样细胞，甚至卵母细胞样细胞（OLC）。然而，这些生殖细胞样细胞（GCLC）是否能产生有活力的后代仍然是未知的。在这篇综述中，我们将讨论来源于GCLC的SDSC的起源和特性，这种分化过程的可能机制，以及SDSC衍生的GCLC的未来生物医学应用。
The skin is a unique organ that contains a variety of stem cells for the maintenance of skin homeostasis and the repair of skin tissues following injury and disease. Skin-derived stem cells (SDSCs) constitute a heterogeneous population of stem cells generated in vitro from dermis, which can be cultured as spherical aggregates of cells in suspension culture. Under certain in vitro or in vivo conditions, SDSCs show multipotency and can generate a variety of neural, mesodermal, and endodermal cell types such as neurons, glia, fibroblasts, adipocytes, muscle cells, chondroblasts, osteoblats, and islet β-cell-like cells. SDSCs are likely derived from multipotent stem cells located in the hair follicles that are, in turn, derived from embryonic migratory neural crest or mesoderm cells. During the past decade, a wave of reports have shown that germ cells can be generated from various types of stem cells. It has been shown that SDSCs are able to produce primordial germ cell-like cells in vitro, and even oocyte-like cells (OLCs). Whether these germ cell-like cells (GCLCs) can give rise to viable progeny remains, however, unknown. In this review, we will discuss the origin and characteristics of SDSCs from which the GCLC are derived, the possible mechanisms of this differentiation process, and finally the prospective biomedical applications of the SDSC-derived GCLCs.