Transcriptional repression directed by the yeast α2 protein in vitro
Transcriptional repression directed by the yeast α2 protein in vitro
复制标题
体外酵母 α2 蛋白指导的转录抑制
作者：
B. M. Herschbach;M. Arnaud;A. Johnson
α2蛋白是一种转录抑制因子，参与出芽酵母（Saccharomyces cerevisiae）细胞类型的调控。α2与血清反应因子相关蛋白Mcm1协同结合到a特异性基因操作子3 - 6上。α2-Mcm1复合体反过来将Ssn6和Tup1招募到操作者，我们认为后两种蛋白负责转录抑制7 - 9。将a-特异性基因操作符放置在测试启动子上游的任何位置都会导致该启动子在体内受到抑制9 - 11。在这方面，a-特异性基因操作符类似于负作用增强子。在这里，我们从远处描述了这个例子的阴性对照的体外重建。我们在体外观察到缺乏外源添加的激活蛋白和缺乏已知激活蛋白结合位点的模板上的抑制，我们推断α2定向的抑制作用于一般的转录机制。
THE α2 protein, a homeodomain protein involved in specifying cell type in the budding yeast Saccharomyces cerevisiae, is a transcriptional represser1,2. α2 binds cooperatively with Mcm1, a serum response factor-related protein, to the a-specific gene operator3–6. The α2-Mcm1 complex in turn recruits Ssn6 and Tup1 to the operator, and we believe that these latter two proteins are responsible for the transcriptional repression7–9. Placement of the a-specific gene operator in any of a variety of positions upstream of a test promoter leads to repression of that promoter in vivo9–11. In this respect, the a-specific gene operator resembles a negatively acting enhancer. Here we describe the in vitro reconstitution of this example of negative control from a distance. We observe repression in vitro in the absence of exogenously added activator protein and on templates that lack binding sites for known activator proteins, and we infer that α2-directed repression acts on the general transcription machinery.
影响因子：
56.9
作者：
J. Sze;M. Woontner;J. A. Jaehning;G. Kohlhaw
通讯作者：
J. Sze;M. Woontner;J. A. Jaehning;G. Kohlhaw
影响因子：
10.5
作者：
SMALL, S;KRAUT, R;LEVINE, M
通讯作者：
LEVINE, M
酿酒酵母全细胞提取物中 RNA 聚合酶 II 的准确引发。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Woontner,M;Jaehning,JA
通讯作者：
Jaehning,JA