Drop-on-demand inkjet-based cell printing with 30-μm nozzle diameter for cell-level accuracy
Drop-on-demand inkjet-based cell printing with 30-μm nozzle diameter for cell-level accuracy
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DOI：
10.1063/1.4968845
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发表时间：
2016-11-01
期刊：
影响因子：
3.2
通讯作者：
Jung, Sungjune
中科院分区：
文献类型：
--
作者：
Kim, Young Kwon;Park, Ju An;Jung, Sungjune
我们提出了按需滴墨的基于喷墨的哺乳动物细胞打印，喷嘴直径为30 μ m，用于细胞级精度。高速成像技术已被用来分析在由压电致动器产生的脉冲压力下喷嘴内的细胞的去和停运动以及喷射后的射流形成。在玻璃基底上的20 × 20点阵列的图案显示，对于非常小的喷嘴，在细胞浓度为5.0 +/-106个细胞ml（-1）时，每个打印的液滴平均包含1.30个细胞，而直径为50和80 μ m的较大喷嘴分别递送2.57和2.88个细胞/液滴。还研究了印刷细胞的大小和浓度对细胞数量的影响。此外，通过分别使用活/死测定试剂盒、CCK-8测定和细胞形态成像检查细胞的活力、增殖和形态，评价了喷嘴直径对打印细胞的影响。我们相信30 μ m的喷墨喷嘴可以用于精确的细胞沉积，而不会对打印的哺乳动物细胞造成任何损害。出版社：AIP Publishing
We present drop-on-demand inkjet-based mammalian cell printing with a 30-mu m nozzle diameter for cell-level accuracy. High-speed imaging techniques have been used to analyze the go-and-stop movement of cells inside the nozzle under a pulsed pressure generated by a piezo-actuator and the jet formation after ejection. Patterning of an array of 20 x 20 dots on a glass substrate reveals that each printed drop contains 1.30 cells on average at the cell concentration of 5.0 +/- 10 6 cells ml(-1) for the very small nozzle, whereas larger nozzles with the diameter of 50 and 80 mu m deliver 2.57 and 2.88 cells per drop, respectively. The effects of the size and concentration of printed cells on the number of cells have also been investigated. Furthermore, the effect of the nozzle diameter on printed cells has been evaluated through an examination of viability, proliferation, and morphology of cells by using a live/dead assay kit, CCK-8 assay, and cellular morphology imaging, respectively. We believe that the 30-mu m inkjet nozzle can be used for precise cell deposition without any damages to the printed mammalian cells. Published by AIP Publishing.