A NEW SENSITIVE AND SPECIFIC BIOASSAY FOR LACTOGENIC HORMONES - MEASUREMENT OF PROLACTIN AND GROWTH-HORMONE IN HUMAN-SERUM
A NEW SENSITIVE AND SPECIFIC BIOASSAY FOR LACTOGENIC HORMONES - MEASUREMENT OF PROLACTIN AND GROWTH-HORMONE IN HUMAN-SERUM
复制标题
DOI：
10.1210/jcem-51-5-1058
复制
发表时间：
1980-01-01
影响因子：
5.8
通讯作者：
FRIESEN, HG
中科院分区：
文献类型：
--
作者：
TANAKA, T;SHIU, RPC;FRIESEN, HG
催乳激素特异性刺激悬浮培养的Nb 2结大鼠淋巴瘤细胞的复制。人催乳素（hPRL）、绵羊、牛和大鼠PRL在10 pg/ml至1 ng/ml的浓度范围内以剂量依赖性方式刺激复制。人、绵羊和牛胎盘催乳素的活性相似。细胞复制由人GH [生长激素]（hGH）刺激，已知其具有催乳活性。检查的其他激素和生长因子无活性。当分别向培养基中加入过量的抗hPRL和抗GH时，hPRL和hGH的生长刺激作用被完全抑制。基于Nb 2结淋巴瘤细胞对催乳激素的反应，开发了生物测定法。人血清刺激细胞复制。如果存在过量的hPRL和hGH抗体，则该效应完全消除。用许多人血清样品获得的刺激与血清中hPRL和hGH浓度的总和良好相关，如通过放射免疫测定法测定的（r = 0.95; P < 0.001）。人血清中hPRL或hGH的浓度可通过加入过量抗hGH或抗hPRL特异性阻断其他激素的生长刺激作用来单独测定。该生物测定法测定血清中PRL和hGH的灵敏度超过放射免疫法。
The replication of Nb 2 Node rat lymphoma cells in suspension culture is specifically stimulated by lactogenic hormones. Human prolactin (hPRL), ovine, bovine and rat PRL stimulated replication in a dose-dependent manner in the concentration range of 10 pg/ml to 1 ng/ml. Human, ovine and bovine placental lactogens were similarly active. Cell replication was stimulated by human GH [growth hormone] (hGH), which is known to have lactogenic activity. Other hormones and growth factors examined were inactive. The growth stimulatory effects of hPRL and hGH were completely inhibited when excess anti-hPRL and anti-GH, respectively, were added to the medium. A bioassay based on the response of the Nb 2 Node lymphoma cells to lactogenic hormones was developed. Human serum stimulated cell replication. The effect was completely abolished if excess antibodies to both hPRL and hGH were present. The stimulation obtained with a number of human serum samples correlated well with the sum of the hPRL and hGH concentrations in the sera, as determined by radioimmunoassay (r = 0.95; P < 0.001). The concentrations of either hPRL or hGH in human serum could be individually determined by specifically blocking the growth stimulatory effect of the other hormone by adding excess anti-hGH or anti-hPRL. The sensitivity of this bioassay for PRL and hGH in serum exceeds that of radioimmunoassay.