Genetic architecture of zinc hyperaccumulation in Arabidopsis halleri: the essential role of QTL x environment interactions
Genetic architecture of zinc hyperaccumulation in Arabidopsis halleri: the essential role of QTL x environment interactions
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DOI：
10.1111/j.1469-8137.2010.03295.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Saumitou-Laprade, Pierre
中科院分区：
文献类型：
--
作者：
Frerot, Helene;Faucon, Michel-Pierre;Saumitou-Laprade, Pierre
本研究旨在确定控制拟南芥锌（Zn）超积累的主要基因组区域，并研究基因型x环境对表型变异的影响。为此，利用种间拟南芥x lyrata peea F-2群体对数量性状位点（qtl）进行了定位。在两种不同锌浓度的土壤上栽培F-2后代和亲本群体的代表。用70个标记构建连锁图谱。在低污染和高污染处理下，锌超积累表现出较高的广义遗传力（分别为81.9%和74.7%）。检测到5个显著的qtl: 2个低污染处理特异性qtl（染色体1和4），3个在两种处理中均鉴定的qtl（染色体3、6和7）。这些qtl分别解释了低污染处理和高污染处理表型变异的50.1%和36.5%。在两个处理中鉴定的两个QTL（染色体3和6）表现出显著的QTL与环境的相互作用。3号染色体上的QTL与先前鉴定的锌和镉（Cd）耐受性主要QTL有很大的共定位。这表明，至少在一定程度上，锌耐受性和超积累具有共同的遗传基础，并且可能是在重金属污染土壤上同时进化的。
P>This study sought to determine the main genomic regions that control zinc (Zn) hyperaccumulation in Arabidopsis halleri and to examine genotype x environment effects on phenotypic variance. To do so, quantitative trait loci (QTLs) were mapped using an interspecific A. halleri x Arabidopsis lyrata petraea F-2 population.The F-2 progeny as well as representatives of the parental populations were cultivated on soils at two different Zn concentrations. A linkage map was constructed using 70 markers.In both low and high pollution treatments, zinc hyperaccumulation showed high broad-sense heritability (81.9 and 74.7%, respectively). Five significant QTLs were detected: two QTLs specific to the low pollution treatment (chromosomes 1 and 4), and three QTLs identified at both treatments (chromosomes 3, 6 and 7). These QTLs explained 50.1 and 36.5% of the phenotypic variance in low and high pollution treatments, respectively. Two QTLs identified at both treatments (chromosomes 3 and 6) showed significant QTL x environment interactions.The QTL on chromosome 3 largely colocalized with a major QTL previously identified for Zn and cadmium (Cd) tolerance. This suggests that Zn tolerance and hyperaccumulation share, at least partially, a common genetic basis and may have simultaneously evolved on heavy metal-contaminated soils.