Multifunctional FePt-Au heterodimers: promising nanotheranostic agents for dual-modality MR/CT imaging diagnosis and in situ cancer therapy
Multifunctional FePt-Au heterodimers: promising nanotheranostic agents for dual-modality MR/CT imaging diagnosis and in situ cancer therapy
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多功能FePt-Au异二聚体：用于双模态MR/CT成像诊断和原位癌症治疗的有前途的纳米治疗剂
DOI：
10.1039/c6ra23645f
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Yu, Dexin
中科院分区：
文献类型：
--
作者：
Wang, Jinlong;Yue, Ludan;Yu, Dexin
我们报道了通过简单的水热法合成多功能FePt-Au杂化纳米颗粒及其在癌症双模态MR/CT成像诊断和同时原位治疗中的潜在应用。FePt-Au HNPs与中-2,3-二巯基琥珀酸（DMSA）和SH-PEG-FA结合后，在生理溶液中具有很高的生物稳定性，并成功靶向叶酸（FA）受体阳性的癌细胞，如MCF-7、HeLa和HepG2。作为ph敏感剂，制备的FePt-Au-DMSA/PEG-FA HNPs由于在细胞内释放的铁诱导产生许多活性氧（ROS）而对靶向癌细胞表现出高的细胞毒性。相应的半最大值抑制浓度值（IC50, Fe）约为3.0 μ g mL（-1）。体外和体内的MR图像和CT扫描表明，HNPs作为一种双模MR/CT成像造影剂具有很大的潜力，可以用于高精度的癌症早期诊断。此外，体内抗肿瘤和组织学研究表明，用HNPs处理后，肿瘤生长明显受到抑制，对其他组织无明显毒性。因此，FePt-Au-DMSA/PEG-FA HNPs是一种很有前途的多功能纳米治疗药物，可用于MR/CT双模成像诊断和原位肿瘤治疗。
We report the synthesis of multifunctional FePt-Au hybrid nanoparticles via a simple hydrothermal approach and their potential application in cancer dual-modality MR/CT imaging diagnosis and simultaneous in situ therapy. After conjugation with meso-2,3-dimercaptosuccinic acid (DMSA) and SH-PEG-FA, the FePt-Au HNPs present high biostability in physiological solutions and successfully target folate acid (FA) receptor-positive cancer cells such as MCF-7, HeLa and HepG2. As a pH-sensitive agent, the as-prepared FePt-Au-DMSA/PEG-FA HNPs exhibit high cytotoxicity to the targeted cancer cells due to the generation of many reactive oxygen species (ROS) induced by the released Fe within the cells. The corresponding half-maximum inhibitory concentration value (IC50, Fe) is about 3.0 mu g mL(-1). MR images and CT scans in vitro and in vivo demonstrate that the HNPs hold great potential as a dual-modality MR/CT imaging contrast agent for high-accuracy early-stage diagnosis of cancer. In addition, in vivo antitumor and histological studies indicate that the tumor growth is significantly inhibited after treating with HNPs with no observable toxicity found in the other tissues. Therefore, the FePt-Au-DMSA/PEG-FA HNPs are a promising multifunctional nanotheranostic agent for dual-modality MR/CT imaging diagnosis and in situ cancer therapy.