Laser desorption ionisation-time of flight mass spectrometry of the tolyporphins, bioactive metabolites from the cyanobacterium Tolypothrix nodosa.
Laser desorption ionisation-time of flight mass spectrometry of the tolyporphins, bioactive metabolites from the cyanobacterium Tolypothrix nodosa.
复制标题
激光解吸电离飞行时间质谱法测定蓝藻 Tolypothrix nodosa 的生物活性代谢物 Tolyporphins。
DOI：
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发表时间：
2011
影响因子：
3.3
通讯作者：
Jonathan Puddick
中科院分区：
文献类型：
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作者：
M. Prinsep;Jonathan Puddick
介绍
甲卟啉是从蓝细菌结节单歧藻（Tolypothrix nodosa）分离的代谢物，其包含具有C-糖苷、氢氧化物或乙酸酯取代基的卟啉样大环。以往的研究表明，卟啉的MALDI/LDI-TOF MS的强自由基的阳离子和阴离子通常观察到的大环断裂的母谱。tolyporphins的光谱和趋势的系列利用部分取代两个新的类似物。
目的
通过LDI-TOF MS检查tolyporphins，并利用观察到的趋势部分地鉴定两种新的类似物。
方法
采用LDI-TOF MS在正离子和负离子模式下对甲苯卟啉进行分析，并采用源后衰减法（LIFT）在正离子模式下对甲苯卟啉进行分析。还通过MALDI-TOF MS分析了Tolyporphin A以进行比较。结果进行了分析，并用于获得两个新的类似物的结构信息。
结果和讨论
所得光谱通常含有强烈的自由基阳离子或阴离子，观察到的大环核心或C-糖苷的碎片很少。这些结果与前人对卟啉的研究结果一致。在LIFT光谱中观察到的主要碎片离子产生了关键的结构信息。两个tolyporphins的不可分离的混合物也进行了检查。通过对母体离子的LIFT谱的分析，推测了这两种新的类似物的结构。
结论
Tolyporphins产生的LDI-TOF质谱有点类似于卟啉的质谱;此外，取代基片段的特征性方式允许部分表征的新类似物Tolyporphins L和M的LDI-TOF质谱与已知的类似物的比较。
INTRODUCTION
The tolyporphins are metabolites isolated from the cyanobacterium Tolypothrix nodosa, comprising a porphyrin-like macrocycle with C-glycoside, hydroxide or acetate substituents. Previous studies of porphyrins by MALDI/LDI-TOF MS indicate that strong radical cations and anions are usually observed in the parent spectra with little fragmentation of the macrocycle. The spectra of the tolyporphins were obtained and trends in the series utilised to partially characterise two new analogues.
OBJECTIVE
To examine tolyporphins by LDI-TOF MS and utilise trends observed to partially characterise two new analogues.
METHODOLOGY
The tolyporphins were analysed by LDI-TOF MS in positive and negative ion mode and by a post source decay method (LIFT) in positive ion mode. Tolyporphin A was also analysed by MALDI-TOF MS for comparison. Results were analysed and used to obtain structural information on two new analogues.
RESULTS AND DISCUSSION
The resulting spectra generally contained intense radical cations or anions, with little fragmentation of the macrocyclic core or the C-glycosides observed. These results are consistent with previous studies of porphyrins. Major fragment ions observed in LIFT spectra yielded key structural information. An inseparable mixture of two tolyporphins was also examined. Analysis of the LIFT spectrum of the parent ion resulted in the postulation of structures of these two new analogues.
CONCLUSIONS
Tolyporphins yield LDI-TOF mass spectra somewhat analogous to those of porphyrins; furthermore, the substituents fragment in a characteristic manner permitting partial characterisation of the new analogues tolyporphins L and M by comparison of their LDI-TOF mass spectra with those of the known analogues.
Tolyporphin（一种新型蓝藻天然产物）逆转多重耐药性。
影响因子：
3.1
作者：
Smith,CD;Prinsep,MR;Caplan,FR;Moore,RE;Patterson,GM
通讯作者：
Patterson,GM