Piezo1 as a force-through-membrane sensor in red blood cells.
Piezo1 as a force-through-membrane sensor in red blood cells.
复制标题
红细胞中作为力穿透膜传感器的 Piezo1。
DOI：
10.7554/elife.82621
复制
发表时间：
2022-12-14
期刊：
影响因子：
7.7
通讯作者：
MacKinnon R
中科院分区：
文献类型：
--
作者：
Vaisey G;Banerjee P;North AJ;Haselwandter CA;MacKinnon R
Piezo 1是红细胞中介导稳态容量控制的牵张激活的Ca 2+通道。在这里，我们研究的组织Piezo 1在红细胞中使用的超分辨率显微镜技术和电子显微镜相结合。Piezo 1在红细胞表面上采用非均匀分布，偏向双凹的“凹坑”。扩散Piezo 1分子，表现出有限的布朗扩散在短时间尺度上和跳跃的长时间尺度上的轨迹，也反映了对酒窝的偏见。这种偏差可以通过压电圆顶的固有曲率和红细胞膜的曲率之间的“曲率耦合”来解释。Piezo 1不与自身形成簇，也不与F-actin、Spectrin或Gardos通道共定位。因此，Piezo 1表现出红细胞中曲率和横向张力的力通过膜传感器的特性。
Piezo1 is the stretch activated Ca2+ channel in red blood cells that mediates homeostatic volume control. Here, we study the organization of Piezo1 in red blood cells using a combination of super-resolution microscopy techniques and electron microscopy. Piezo1 adopts a non-uniform distribution on the red blood cell surface, with a bias toward the biconcave ‘dimple’. Trajectories of diffusing Piezo1 molecules, which exhibit confined Brownian diffusion on short timescales and hopping on long timescales, also reflect a bias toward the dimple. This bias can be explained by ‘curvature coupling’ between the intrinsic curvature of the Piezo dome and the curvature of the red blood cell membrane. Piezo1 does not form clusters with itself, nor does it colocalize with F-actin, Spectrin, or the Gardos channel. Thus, Piezo1 exhibits the properties of a force-through-membrane sensor of curvature and lateral tension in the red blood cell.