Proteomic characterization of primary diffuse large B-cell lymphomas in the central nervous system.
Proteomic characterization of primary diffuse large B-cell lymphomas in the central nervous system.
复制标题
中枢神经系统原发性弥漫性大 B 细胞淋巴瘤的蛋白质组学特征。
作者：
Jie Li;H. Okamoto;Chunyue Yin;J. Jagannathan;J. Takizawa;S. Aoki;S. Gläsker;E. Rushing;A. Vortmeyer;E. Oldfield;R. Yamanaka;Z. Zhuang
对象
原发性中枢神经系统淋巴瘤（PCNSL）的发病机制是中枢神经系统（CNS）内缺乏原发性淋巴组织的一个难题。比较PCNSL和散发性系统性淋巴瘤（SSL）的蛋白表达提供了一个有用的策略，用于识别表征疾病相关特征的分子特征，并提供有关肿瘤发生和进展的信息。
方法
选择7个弥漫性大B细胞PCNSL进行2D凝胶电泳，并从这些PCNSL的蛋白质组进行了比较，从7个弥漫性大B细胞SSLs。使用质谱法对区分蛋白质进行测序。
结果
二维凝胶电泳从每个标本中鉴定出平均706种蛋白质。计算机凝胶分析和人工再确认显示PCNSL和SSLs的蛋白质组具有96%的相似性。比较分析鉴定了9个蛋白在PCNSL中显著过表达（p < 0.05），16个蛋白在PCNSL中下调。蛋白质组学研究结果进一步验证使用蛋白质印迹和免疫组化染色。
结论
PCNSL和SSL之间蛋白质组模式的相似性表明，这些肿瘤类型具有在分化过程中获得的结构相似性。淋巴瘤细胞的最终命运（中枢神经系统与系统）可能与差异表达的蛋白质有关，这些蛋白质在归巢和宿主加工中起作用。阐明这些差异表达蛋白的作用，将被证明是有价值的理解PCNSL的发病机制。
OBJECT
The lack of primary lymphoid tissue within the central nervous system (CNS) confounds our understanding of the pathogenesis of primary CNS lymphomas (PCNSLs). Comparing the protein expression of PCNSLs and sporadic systemic lymphomas (SSLs) provides a useful strategy for identifying a molecular signature that characterizes disease-associated features and provides information regarding tumor initiation and progression.
METHODS
Seven diffuse large B-cell PCNSLs were selected to undergo 2D gel electrophoresis, and profiled proteomes from these PCNSLs were compared with those from 7 diffuse large B-cell SSLs. Distinguishing proteins were sequenced using mass spectrometry.
RESULTS
Two-dimensional gel electrophoresis identified an average of 706 proteins from each specimen. Computerized gel analysis and manual reconfirmation revealed a 96% similarity in the proteomes of PCNSLs and SSLs. Comparative analysis identified 9 proteins significantly overexpressed (p < 0.05) and 16 proteins downregulated in PCNSLs. The proteomic findings were further validated using Western blot and immunohistochemical staining.
CONCLUSIONS
The similarities in proteomic patterns between PCNSLs and SSLs suggest that these tumor types share structural similarities, acquired during differentiation. The ultimate fate of lymphomatous cells (CNS vs systemic) may be related to differentially expressed proteins, which function in homing and host processing. Elucidating the roles of these differentially expressed proteins will prove valuable in understanding the pathogenesis of PCNSL.
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影响因子：
56.9
作者：
CHIANG, HL;TERLECKY, SR;DICE, JF
通讯作者：
DICE, JF
增加 WEHI7.2 细胞的抗氧化防御能力会产生更像肿瘤的代谢特征。
DOI：
--
发表时间：
2005
期刊：
International journal of molecular medicine.
影响因子：
--
作者：
Tome,MargaretE;Briehl,MargaretM;Lutz,NorbertW
通讯作者：
Lutz,NorbertW
DOI：
10.1073/pnas.90.9.4112
发表时间：
1993-05-01
影响因子：
11.1
作者：
MANDEL, R;RYSER, HJP;PEAK, D
通讯作者：
PEAK, D
DOI：
--
发表时间：
1990-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Feener;Wei-Chiang Shen;Hugues J.-P. Ryser
通讯作者：
E. Feener;Wei-Chiang Shen;Hugues J.-P. Ryser
原发性中枢神经系统和原发性眼内淋巴瘤的分子分析。
影响因子：
2.5
作者：
Tuaillon,N;Chan,CC
通讯作者：
Chan,CC