Cell-cell communication, chemotaxis and recruitment in Vibrio parahaemolyticus.
Cell-cell communication, chemotaxis and recruitment in Vibrio parahaemolyticus.
复制标题
副溶血弧菌的细胞间通讯、趋化性和募集。
DOI：
10.1111/mmi.14256
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发表时间：
2019
影响因子：
3.6
通讯作者：
McCarter,LindaL
中科院分区：
文献类型：
--
作者：
Lamb,Evan;Trimble,MichaelJ;McCarter,LindaL
运动细菌擅长寻找最佳定植环境。通常，它们利用趋化性来感知营养物质的可用性和有毒化学物质的危险浓度。对于许多细菌来说，化学感受器的种类很多，这表明它们在感知方面拥有广泛的味觉。然而，对趋化信号转导系统检测到的分子的了解是有限的。有些细菌，如副溶血弧菌，是群居性的，在表面成群聚集。这种海洋细菌和人类病原体会分泌 S 信号自诱导子，该信号会提示细胞内 c-di-GMP 的降解，从而导致集群程序的转录。在这里，我们报告说，S 信号还通过充当化学引诱剂在行为水平上引导运动。数据表明V.副溶血弧菌使用 SscL 和 SscS（同源甲基接受趋化蛋白）感知 S 信号。浮游细菌需要 SscL 来实现 S 信号趋化。 SscS在集群过程中发挥作用，缺乏这种化学感受器的突变体集群速度更快，并产生比野生型或sscL突变体具有更深分支的集群前沿的集落。其他弧菌物种可以向 S 信号游动，这表明这种细胞间通讯分子在多微生物海洋群落中具有招募作用。
Motile bacteria are proficient at finding optimal environments for colonization. Often, they use chemotaxis to sense nutrient availability and dangerous concentrations of toxic chemicals. For many bacteria, the repertoire of chemoreceptors is large, suggesting they possess a broad palate with respect to sensing. However, knowledge of the molecules detected by chemotaxis signal transduction systems is limited. Some bacteria, likeVibrio parahaemolyticus, are social and swarm in groups on surfaces. This marine bacterium and human pathogen secretes the S signal autoinducer, which cues degradation of intracellular c‐di‐GMP leading to transcription of the swarming program. Here, we report that the S signal also directs motility at a behavioral level by serving as a chemoattractant. The data demonstrate thatV. parahaemolyticussenses the S signal using SscL and SscS, homologous methyl‐accepting chemotaxis proteins. SscL is required by planktonic bacteria for S signal chemotaxis. SscS plays a role during swarming, and mutants lacking this chemoreceptor swarm faster and produce colonies with more deeply branched swarming fronts than the wild type or thesscLmutant. OtherVibriospecies can swim toward the S signal, suggesting a recruitment role for this cell‐cell communication molecule in the context of polymicrobial marine communities.