Axonal Transport of the Molecular Forms of Acetylcholinesterase in Chick Sciatic Nerve
Axonal Transport of the Molecular Forms of Acetylcholinesterase in Chick Sciatic Nerve
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乙酰胆碱酯酶分子形式在鸡坐骨神经中的轴突运输
DOI：
10.1111/j.1471-4159.1980.tb07859.x
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发表时间：
1980
影响因子：
4.7
通讯作者：
L. Giamberardino
中科院分区：
文献类型：
--
作者：
J. Couraud;L. Giamberardino
采用蔗糖梯度沉降法研究了4周龄来航鸡坐骨神经乙酰胆碱酯酶（AChE）的多态性。AChE主要有4种分子形式，沉降系数分别为5S、7.5S、11.5S和20 S。轴突运输的每一个这些形式进行了研究的基础上的酶积累动力学两侧的神经横切和酶的再分布动力学在体内分离的神经节段。神经切断后，11.5S和20 S型在顺行方向的积累比逆行方向快，也比5S和7.5S型在顺行方向快得多。5S和7.5S的逆行累积微弱或可忽略不计。此外，神经切断后1小时，11.5S和20 S型（但不是5S和7.5S型）的蓄积率在两个方向上都下降到其初始值的约三分之一，这可能是由于轴突切断部位的轴突运输逆转。放线菌酮的局部蛋白质合成抑制并不影响11.5S和20 S在横切前的积累，至少在最初的几个小时内，但减少了约40%的5S和7.5S。在离体神经节段在体内，迅速移动的部分的乙酰胆碱酯酶估计构成的总酶活性存在于神经中，14%，它在顺行和9%在逆行方向移动。少量11.5S分子（约）20%）是快速移动（三分之二在顺行方向，三分之一在逆行方向），而几乎所有的20 S-约90%-快速迁移（三分之二向前，三分之一向后）。11.5S型和20 S型的顺行速度分别为408 ± 94和411 ± 161 mm/天。逆行速度分别为175 ± 85和145 ± 107 mm/d。假设5S和7.5S分子的总量沿顺行方向移动，则它们的累积速率分别与平均顺行速度2.9 ± 1.3和5.1 ± 1.4 mm/天一致。库洛和Di Giamberardino L.乙酰胆碱酯酶分子形式在鸡坐骨神经中的轴突转运。神经化学杂志。35，1053-1066（1980）中描述的。
Acetylcholinesterase (AChE) polymorphism was studied in the sciatic nerve of 4‐week‐old Leghorn chicks, by sucrose gradient sedimentation analysis. Four main AChE molecular forms were found with sedimentation coefficients of 5S, 7.5S, 11.5S and 20S respectively. Axonal transport of each of these forms was investigated on the basis of the enzyme accumulation kinetics measured on both sides of nerve transections and of the enzyme redistribution kinetics in nerve segments isolated in vivo. After nerve transection, 11.5S and 20S forms accumulated faster in the anterograde than in the retrograde direction and also much faster than 5S and 7.5S forms in the anterograde direction. Retrograde accumulations of 5S and 7.5S were faint or negligible. In addition, 1 h after nerve cutting, the accumulation rates for 11.5S and 20S forms (but not for 5S and 7.5S) fell, in both directions, to about one‐third of their initial values, probably owing to reversal of axonal transport at the axotomy site. Local protein synthesis inhibition by cycloheximide did not affect the accumulation of 11.5S and 20S in front of a transection, at least during the first hours, but reduced that of 5S and 7.5S by about 40%. In isolated nerve segments in vivo, the rapidly mobile fraction of AChE was estimated to constitute 23% of the total enzyme activity present in the nerve, 14% of it moving in an anterograde and 9% in a retrograde direction. A small amount of 11.5S molecules (approx. 20%) was in rapid transit (two‐thirds in the anterograde and one‐third in the retrograde direction), whereas almost all the 20S—about 90%—migrated rapidly (two‐thirds forwards and one‐third backwards). Anterograde velocities of 408 ± 94 and 411 ± 161 mm/day respectively were estimated for the 11.5S and 20S forms. Their respective retrograde velocities were 175 ± 85 and 145 ± 107 mm/day. Assuming that the totality of 5S and 7.5S molecules are moving in the anterograde direction, their accumulation rates were consistent with the average anterograde velocities of 2.9 ± 1.3 and 5.1 ± 1.4 mm/day, respectively. Couraud J. Y. and Di Giamberardino L. Axonal transport of the molecular forms of acetylcholinesterase in chick sciatic nerve. J. Neurochem. 35, 1053–1066 (1980).