Monolithically fabricated perforated polyimide separator for a planar lipid bilayer devi CE
Monolithically fabricated perforated polyimide separator for a planar lipid bilayer devi CE
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用于平面脂质双层器件 CE 的整体制造的穿孔聚酰亚胺隔膜
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
S. Takeuchi
中科院分区：
文献类型：
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作者：
T. Osaki;M. Kaneko;K. Araki;H. Uehara;T. Ura;H. Hirata;K. Kamiya;S. Fujii;N. Misawa;S. Takeuchi
我们提出了一种批量制造穿孔分离器以形成平面脂质双层的方法，旨在实现商业和大规模生产。平面脂质双层体系作为基于纳米孔的生物传感器的平台越来越受到人们的关注，而穿孔分离器是在此类传感器上重建平面双层的重要组成部分。因此，我们设计了一种大规模制造分离器的程序，避免了繁琐的工艺。首先，用化学腐蚀的方法在聚酰亚胺薄膜(200×200mm2)上加工了微孔阵列。然后将光反应聚酰亚胺覆盖物层叠，并在薄膜的两面形成一个开孔的框架作为增强。通过该工艺获得了约300块整体分离器。用研制的分离器验证了平面双层膜的电学性能(电流噪声水平)。
We propose a batch fabrication of a perforated separator for formation of a planar lipid bilayer, aiming for commercial and mass production. A planar lipid-bilayer system increasingly attracts attention as a platform of nanopore-based biosensors, and a perforated separator is an essential component for reconstitution of a planar bilayer on such sensor devices. We therefore designed a mass-fabrication procedure of the separator, avoiding laborious processes. First, an array of microapertures was processed on a sheet of polyimide thin film (200 × 200 mm2) by chemical etching. Photo-reactive polyimide coverlays were then laminated and formed a frame of the apertures at both faces of the film as reinforcement. Approximately 300 pieces of monolithic separators were obtained by the procedure. Electrical properties (current noise level) of a planar bilayer were certified with the developed separator.