Neuroprotective effects of curculigoside against NMDA-induced neuronal excitoxicity in vitro
Neuroprotective effects of curculigoside against NMDA-induced neuronal excitoxicity in vitro
复制标题
仙茅苷对 NMDA 诱导的神经元兴奋毒性的体外神经保护作用
DOI：
10.1016/j.fct.2012.08.006
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发表时间：
2012-11-01
影响因子：
4.3
通讯作者：
Liu, Shui-bing
中科院分区：
文献类型：
--
作者：
Tian, Zhen;Yu, Wei;Liu, Shui-bing
谷氨酸是中枢神经系统中一种重要的兴奋性神经递质。谷氨酸的过度积累可引起兴奋性毒性，在脊髓损伤、外伤性脑损伤、中风和神经退行性疾病中起关键作用。莪术苷（Curculigo orchioides， CCGS）是从莪术根中分离得到的主要生物活性化合物。CCGS具有广泛的生物学效应，已广泛应用于中药中。然而，CCGS对谷氨酸引起的兴奋性毒性的神经保护作用知之甚少。本研究旨在评价CCGS对培养皮层神经元的神经保护作用。结果表明，1 μ M和10 μ M CCGS对n-甲基-d -天冬氨酸（NMDA）诱导的神经元细胞损失有明显的预防作用，并呈时间和浓度依赖性地减少了凋亡和坏死细胞的数量。CCGS的神经保护作用与下调培养皮层神经元细胞凋亡蛋白水平和减少细胞内活性氧的产生有关。这些发现为天然抗兴奋毒性药物的开发提供了新的思路。(C) 2012 Elsevier Ltd.版权所有。
Glutamate is an important excitatory neurotransmitter in the central nervous system. Excessive accumulation of glutamate can cause excitotoxicity, which plays a key role in spinal cord injury, traumatic brain injury, stroke, and neurodegenerative diseases. Curculigoside (CCGS) is a major bioactive compound isolated from the rhizome of Curculigo orchioides Gaertn. CCGS has an extensive biological effect and has been used in Traditional Chinese Medicine. However, little is known about the neuroprotective effects of CCGS on glutamate-induced excitotoxicity. This study aims to evaluate the neuroprotective effects of CCGS in cultured cortical neurons. The results indicated that treatment with 1 and 10 mu M CCGS evidently prevented N-methyl-D-aspartate (NMDA)-induced neuronal cell loss and reduced the number of apoptotic and necrotic cells in a time- and concentration-dependent manner. The neuroprotective effects of CCGS are related to down regulating the apoptotic protein levels and reducing the production of intracellular reactive oxygen species in cultured cortical neurons. These findings give a new insight into the development of natural anti-excitotoxicity agents. (C) 2012 Elsevier Ltd. All rights reserved.