The hypo-osmotic swelling test for evaluation of sperm membrane integrity.
The hypo-osmotic swelling test for evaluation of sperm membrane integrity.
复制标题
用于评估精子膜完整性的低渗透膨胀试验。
DOI：
10.1007/978-1-62703-038-0_3
复制
发表时间：
2013
影响因子：
--
通讯作者：
R. Jeyendran
中科院分区：
文献类型：
--
作者：
S. Ramu;R. Jeyendran
功能膜对于精子的可复性是必需的，因为它在精子获能、顶体反应和精子与卵表面的结合中起着不可或缺的作用。低渗肿胀（HOS）试验评估精子质膜的功能完整性，也是精子生育能力的有用指标。HOS测试通过确定精子膜维持精子细胞与其环境之间平衡的能力来预测膜完整性。由于低渗压力引起的液体流入导致精子尾部卷曲和气球状或“膨胀”。“肿胀精子的百分比较高表明精子具有功能性和完整的质膜。在这里，我们提出了执行HOS测试的详细协议，并解释了解释的结果。
A functional membrane is requisite for the fertilizing ability of spermatozoa, as it plays an integral role in sperm capacitation, acrosome reaction, and binding of the spermatozoon to the egg surface. The hypo-osmotic swelling (HOS) test evaluates the functional integrity of the sperm's plasma membrane and also serves as a useful indicator of fertility potential of sperm. The HOS test predicts membrane integrity by determining the ability of the sperm membrane to maintain equilibrium between the sperm cell and its environment. Influx of the fluid due to hypo-osmotic stress causes the sperm tail to coil and balloon or "swell." A higher percentage of swollen sperm indicates the presence of sperm having a functional and intact plasma membrane. Here, we present the detailed protocol for performing the HOS test and explain the results for interpretation.